Fabrication of large-scale gold nanoplate films as highly active SERS substrates for label-free DNA detection
Fabrication of large-scale gold nanoplate films as highly active SERS substrates for label-free DNA detection
复制标题
制备大规模金纳米板薄膜作为高活性 SERS 基底，用于无标记 DNA 检测
DOI：
10.1016/j.bios.2012.11.029
复制
发表时间：
2013-05-15
影响因子：
12.6
通讯作者：
Qian, Weiping
中科院分区：
文献类型：
--
作者：
Bi, Liyan;Rao, Yanying;Qian, Weiping
We introduce a simple but robust label-free method to detect DNA based on large-scale gold nanoplate (GNP) films with tunable localized surface plasmon resonance (LSPR) and highly surface-enhanced Raman scattering (SERS) activity. The common probe molecule, Neil Blue A sulfate (NBA) is used for testing the SERS activity of the GNP films at very low concentrations. It is found that the SERS properties are highly dependent on the edge lengths of gold nanoplate and gold nanoplate density in the films. Multiple-layer GNP films which are constructed by gold nanoplate with an edge length of 134 +/- 6 nm have the density of 916 +/- 40 GNPsGNPs/spot. It shows the highest signal intensity with SERS enhancement factor (EF) as high as 5.4 x 10(7) and also has excellent stability, reproducibility and repeatability. The optimized SERS-active substrate with the largest enhancement ability could be used to detect double-strand DNA without a dye label, and the detection limit is down to 10(-6) mg/mL. (c) 2012 Elsevier B.V. All rights reserved.
