Effects of mitogenic hormones on HSP70 expression in a silver sea bream fibroblast cell line and a primary macrophage preparation
Effects of mitogenic hormones on HSP70 expression in a silver sea bream fibroblast cell line and a primary macrophage preparation
复制标题
DOI：
10.1016/j.ygcen.2006.12.020
复制
发表时间：
2007-06-01
影响因子：
2.7
通讯作者：
Woo, Norman Y. S.
中科院分区：
文献类型：
--
作者：
Deane, Eddie E.;Zhou, Liran;Woo, Norman Y. S.
本研究的目的是阐明三种促有丝分裂激素[生长激素（GH）、胰岛素样生长因子-1（IGF-1）和催乳素（PRL）]对银鲷成纤维细胞系和原代巨噬细胞制剂中热休克蛋白70（HSP70）表达的作用和重要性。暴露于浓度为1-1000 ng/ml的GH的成纤维细胞和巨噬细胞没有表现出受调节的HSP70表达，而暴露浓度为10 ng/ml的GH降低了巨噬细胞中的HSP70水平。暴露于IGF-1后，发现在暴露浓度为1-10ng/ml时，成纤维细胞中HSP70表达保持不变，但巨噬细胞中HSP70表达显着降低。最后，使用羊PRL制剂发现，在1-1000ng/ml的暴露浓度下，成纤维细胞中的HSP70表达降低，并且在1-100ng/ml的暴露浓度下，巨噬细胞中的HSP70表达也降低。 (c) 2006 Elsevier Inc. 保留所有权利。
This study was performed in order to elucidate the role and importance of three mitogenic hormones [growth hormone (GH), insulin-like growth factor-1 (IGF-1) and prolactin (PRL)] on heat shock protein 70 (HSP70) expression in a silver sea bream fibroblast cell line and a primary macrophage preparation. Fibroblasts and macrophages that were exposed to GH at concentrations of 1-1000 ng/ml did not exhibit modulated HSP70 expression, whereas GH at an exposure concentration of 10 ng/ml lowered HSP70 levels in macrophages. Upon exposure to IGF-1 it was found that HSP70 expression remained unchanged in fibroblasts but was significantly decreased in macrophages at exposure concentrations of 1-10ng/ml. Finally, and using a preparation of ovine PRL it was found that HSP70 expression decreased in fibroblasts at exposure concentrations of 1-1000 ng/ml and also decreased in macrophages at exposure concentrations of 1-100 ng/ml. (c) 2006 Elsevier Inc. All rights reserved.