LEVELS OF RIBOSOMAL PROTEIN-S1 AND ELONGATION FACTOR-G IN THE GROWTH-CYCLE OF ESCHERICHIA-COLI
LEVELS OF RIBOSOMAL PROTEIN-S1 AND ELONGATION FACTOR-G IN THE GROWTH-CYCLE OF ESCHERICHIA-COLI
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DOI：
10.1128/jb.154.3.1323-1328.1983
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发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
TRAUT, RR
中科院分区：
文献类型：
--
作者：
LAMBERT, JM;BOILEAU, G;TRAUT, RR
用双向凝胶电泳法检测核糖体、核糖体蛋白S1和延伸因子G[EF-G]在大肠杆菌生长周期中的相对水平。第一维采用非平衡pH梯度聚丙烯酰胺凝胶电泳法，第二维采用聚丙烯酰胺梯度-十二烷基硫酸钠凝胶电泳法。通过蛋白质的放射性碘化和标记多肽的多肽图谱，确认了含有S1和EF-G的蛋白质斑点的同一性。核糖体和核糖体蛋白S1水平在指数期向稳定期转变过程中协调下降。固定相中未见S1的积累。从指数期到稳定期，EF-G水平无明显变化。与核糖体或S1相比，EF-G的相对水平增加.apprx。在从指数相到稳定相的过渡过程中增加了2.5倍。因此，在从指数期到稳定期的转变过程中，EF-G和包括S1在内的核糖体蛋白水平的调节是不同的。
The relative levels of ribosomes, ribosomal protein S1 and elongation factor G [EF-G)] in the growth cycle of E. coli were examined with 2-dimensional polyacrylamide gel electrophoresis. Nonequilibrium pH gradient polyacrylamide gel electrophoresis was used in the 1st dimension and polyacrylamide gradient-sodium dodecyl sulfate gel electrophoresis was used in the 2nd dimension. The identities of protein spots containing S1 and EF-G were confirmed by radioiodination of the proteins and peptide mapping of the radiolabeled peptides. The levels of ribosomes and ribosomal protein S1 were coordinately reduced during transition from exponential phase to stationary phase. There was no accumulation of S1 in the stationary phase. The level of EF-G showed no significant change from exponential phase to stationary phase. The relative level of EF-G compared with ribosomes or S1 increased .apprx. 2.5-fold during transition from exponential phase to stationary phase. Thus, there are differences between the regulation of the levels of EF-G and of ribosomal proteins, including S1, during the transition from exponential to stationary phase.