Effect of gamma-irradiation on the effector function of T lymphocytes in microbial control.
Effect of gamma-irradiation on the effector function of T lymphocytes in microbial control.
复制标题
微生物控制中伽马射线照射对 T 淋巴细胞效应功能的影响。
DOI：
10.1080/09553009514550671
复制
发表时间：
1995
影响因子：
2.6
通讯作者：
Rayford,PL
中科院分区：
文献类型：
--
作者：
Igietseme,JU;Smith,K;Simmons,A;Rayford,PL
本研究利用一种在体内和体外均能抑制感染性衣原体生长的衣原体特异性T细胞克隆，研究了γ射线照射对效应T细胞控制感染能力的影响。用不同剂量（0、2·5、5·0、10·0、20·0和40·0戈伊）照射小鼠肺炎沙眼衣原体生物变种（MoPn）特异性克隆2.14-0（CD 4+），观察其生物学功能和抑制MoPn上皮内生长的能力。结果表明，γ射线照射虽然显著降低了克隆对抗原的增殖反应，但并不影响克隆的表型和抑制衣原体生长的效应功能。γ射线照射后抗衣原体功能的保留与抗原刺激后IFN-γ和TNF-α分泌的保留相关。我们的结论是，需要增殖和分化的T细胞的生物学功能更可能受到γ射线照射的短期不利影响，但效应功能，可能与细胞因子和细胞溶解，可能会保留在持续的效应T细胞在照射的个人。
A chlamydial-specific T cell clone, capable of inhibiting the growth of infectiousChlamydia in vivoandin vitro, was employed to investigate the effect of γ-irradiation on the ability of effector T cells to control infections. Clone 2.14-0 (CD4+), specific for theChlamydia trachomatisbiovar agent of mouse pneumonitis (MoPn), was irradiated with varying doses (0, 2·5, 5·0, 10·0, 20·0 and 40·0 Gy) and its biological functions and ability to inhibit the intraepithelial growth of MoPn were assessed. The results revealed that although γ-irradiation drastically reduced the proliferative response of the clone to antigen, it did not affect the phenotype and the effector function of inhibiting chlamydial growth. The preservation of anti-chlamydial function after γ-irradiation correlated with the retention of IFN-γ and TNF-α secretion in response to antigenic stimulation. We conclude that the biologic functions of T cells requiring proliferation and differentiation are more likely to be adversely affected by γ-irradiation on the short-term, but the effector functions, possibly associated with cytokines and cytolysis, may be preserved among persisting effector T cells in an irradiated individual.