Exploratory Full-Field Strain Analysis of Regenerated Bone Tissue from Osteoinductive Biomaterials
Exploratory Full-Field Strain Analysis of Regenerated Bone Tissue from Osteoinductive Biomaterials
复制标题
骨诱导生物材料再生骨组织的探索性全场应变分析
DOI：
10.3390/ma13010168
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发表时间：
2020-01-01
期刊：
影响因子：
3.4
通讯作者：
Tozzi, Gianluca
中科院分区：
文献类型：
--
作者：
Fernandez, Marta Pena;Black, Cameron;Tozzi, Gianluca
用于骨再生的生物材料正在不断开发中，它们在临界尺寸缺损中的应用代表了骨移植技术的一种有前途的替代方法。然而，所有这些材料产生与天然组织机械相当的骨的能力仍然不清楚。本研究旨在探索通过不同的骨诱导策略（包括释放BMP-2的递送系统）在体内产生的新形成的骨组织中的全场应变演变。原位高分辨率X射线显微计算机断层扫描（microCT）和数字体积相关（DVC）被用来定性评估再生骨组织的微观力学。在单个时间点（体内10周）取出的标本（n = 2 p/治疗）中，评价了与不同骨形态测定和矿化相关的组织局部应变。结果表明，不同的治疗方法具有不同的载荷传递能力，突出了骨愈合早期骨结构的机械适应性。尽管由于样本量有限而具有探索性，但本文报告的结果和分析表明，microCT和DVC的组合如何能够增强对体内新形成骨的微观力学的理解，并有可能为进一步开发新型骨再生方法提供信息。
Biomaterials for bone regeneration are constantly under development, and their application in critical-sized defects represents a promising alternative to bone grafting techniques. However, the ability of all these materials to produce bone mechanically comparable with the native tissue remains unclear. This study aims to explore the full-field strain evolution in newly formed bone tissue produced in vivo by different osteoinductive strategies, including delivery systems for BMP-2 release. In situ high-resolution X-ray micro-computed tomography (microCT) and digital volume correlation (DVC) were used to qualitatively assess the micromechanics of regenerated bone tissue. Local strain in the tissue was evaluated in relation to the different bone morphometry and mineralization for specimens (n = 2 p/treatment) retrieved at a single time point (10 weeks in vivo). Results indicated a variety of load-transfer ability for the different treatments, highlighting the mechanical adaptation of bone structure in the early stages of bone healing. Although exploratory due to the limited sample size, the findings and analysis reported herein suggest how the combination of microCT and DVC can provide enhanced understanding of the micromechanics of newly formed bone produced in vivo, with the potential to inform further development of novel bone regeneration approaches.