Antibiotic-resistant Staphylococcus aureus isolated from milk in the Mafikeng Area, North West province, South Africa
Antibiotic-resistant Staphylococcus aureus isolated from milk in the Mafikeng Area, North West province, South Africa
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DOI：
10.4102/sajs.v106i11/12.243
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发表时间：
2010-12-01
影响因子：
2.4
通讯作者：
Bezuidenhout, CC
中科院分区：
文献类型：
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作者：
Ateba, CN;Mbewe, M;Bezuidenhout, CC
本研究的目的是分离金黄色葡萄球菌的牛奶样品从不同的农场设置，并确定其抗生素敏感性模式。采用革兰氏染色、氧化酶、过氧化氢酶、DNA酶、溶血和MASTASTAPH（TM）快速凝集试验进行细菌鉴定。共收集28份牛奶样品，并筛选是否存在S。金黄色。所有样品均被S.金黄色。共240个S。在本研究期间获得金黄色葡萄球菌分离株。S.与Rooigrond和Molelwane的商业农场（分别为17.9%和22.1%）相比，Lokaleng和Mogosane的公共农场（分别为24.6%和35.4%）获得的牛奶中的金黄色葡萄球菌含量更高。大部分的S。两个农场的金黄色葡萄球菌对甲氧西林（MT）、氨苄西林（AP）、青霉素G（PG）、磺胺甲恶唑（Smx）、土霉素（OT）、红霉素（E）、呋喃妥因（NI）和链霉素（S）耐药率为39%~ 100%，但对万古霉素（V）不耐药。对磺胺甲恶唑和呋喃妥因的耐药率更高（64.2% - 100%）。来自两个公共农场的分离株中对万古霉素耐药的百分比略低（3.4% - 4.7%），但来自商业农场牛奶的所有分离株均对该药物敏感。来自商业农场的分离株的主要多重抗生素耐药表型分别为Rooigrond和Molelwane农场的AP-Smx-NI和MT-AP-PG-OT-Smx-NI，而来自公共农场的分离株的主要多重抗生素耐药表型分别为Lokaleng和Mogosane的MT-AP-PG-Smx-E-NI-S和MT-AP-PG-OT-Smx-NI-S。比较商业农场分离株的抗生素耐药性百分比，发现商业农场分离株之间呈显著正相关（r = 0.966，p < 0.01）。S.金黄色葡萄球菌通常存在于人类中，因此，S.奶牛体内存在的金黄色葡萄球菌可能是由两个物种之间的传播造成的，强调需要改善挤奶环境的卫生条件。
The aim of this study was to isolate Staphylococcus aureus from samples of cow's milk obtained from different farm settings and to determine their antibiotic susceptibility patterns. Gram staining, oxidase, catalase, DNase, haemolysis and the MASTASTAPH (TM) rapid agglutination tests were employed for bacterial identification. A total of 28 milk samples were collected and screened for the presence of S. aureus. All the samples were contaminated with S. aureus. A total of 240 S. aureus isolates were obtained during this study. The levels of contamination with S. aureus were higher in milk obtained from the communal farms in Lokaleng and Mogosane (24.6% and 35.4%, respectively) compared to the commercial farms in Rooigrond and Molelwane (17.9% and 22.1%, respectively). A large percentage of the S. aureus isolates (39%-100%) from both communal farms was resistant to methicillin (MT), ampicillin (AP), penicillin G (PG), sulphamethoxazole (Smx), oxytetracycline (OT), erythromycin (E), nitrofurantoin (NI) and streptomycin (S), but not vancomycin (V). An even higher percentage (64.2% - 100%) of the isolates from both commercial farms was resistant to sulphamethoxazole and nitrofurantoin. A comparably smaller percentage (3.4% - 4.7%) of the isolates from both communal farms was resistant to vancomycin, but all isolates from commercial farm milk were susceptible to this drug. The predominant multiple antibiotic resistant phenotypes for isolates from the commercial farms were AP-Smx-NI and MT-AP-PG-OT-Smx-NI for Rooigrond and Molelwane farms, respectively, while those for isolates from the communal farms were MT-AP-PG-Smx-E-NI-S and MT-AP-PG-OT-Smx-NI-S for Lokaleng and Mogosane, respectively. When comparing the percentage of antibiotic resistance, a significant positive correlation was observed between the isolates from the commercial farms (r = 0.966,p < 0.01). S. aureus is normally resident in humans, therefore, the S. aureus present in the cows may have resulted from transmission between the two species, emphasising the need to improve sanitary conditions in the milking environment.