P4 down-regulates Jagged2 and Notch1 expression during primordial folliculogenesis.
P4 down-regulates Jagged2 and Notch1 expression during primordial folliculogenesis.
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DOI：
10.2741/e579
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发表时间：
2012-06
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影响因子：
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通讯作者：
Meng Guo;Hua Zhang;Fenghua Bian;Ge Li;Xinyi Mu;Jing Wen;Guankun Mao;Zhen Teng;G. Xia;Meijia Zhang
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作者：
Meng Guo;Hua Zhang;Fenghua Bian;Ge Li;Xinyi Mu;Jing Wen;Guankun Mao;Zhen Teng;G. Xia;Meijia Zhang
孕激素（P4）和Notch信号抑制剂可以抑制小鼠卵巢的巢破裂和原始卵泡发生。然而，这两个信号之间的关系在这个过程中仍然未知。在本研究中，转录水平的锯齿状蛋白2，刻缺蛋白1，和他们的目标，Hey2，在卵巢卵泡形成的开始阶段（17.5天后性交（dpc）出生）显着增加。母亲的P4水平同时下降。我们发现，母体妊娠中期P4水平显着抑制Jagged2，Notch1，Hey2的表达，和卵泡形成在体外。通过每天注射维持高的母体P4水平也显著抑制了Jagged2、Notch1和Hey2的表达，以及妊娠后期的卵泡形成。基于免疫组化，Jagged2定位于卵母细胞和Notch1强烈染色的前颗粒细胞在19.5 dpc卵巢。通过添加抗体和RNAi抑制它们的功能显著抑制巢破裂和卵泡形成。总之，这些结果表明，在妊娠中期的母体P4水平可以抑制Jagged2和Notch1的表达，这是参与原始卵泡发生，在小鼠胎儿卵巢。
Nest breakdown and primordial folliculogenesis of the mouse ovary can be inhibited by progesterone (P4) and Notch signaling inhibitors. However, the relationship between these two signals during this process remains unknown. In the present study, transcript levels of Jagged2, Notch1, and their target, Hey2, increased markedly in ovaries during the beginning stage of folliculogenesis (17.5 days post coitus (dpc) to birth). Maternal P4 levels decreased simultaneously. We found that maternal midpregnancy P4 levels significantly inhibited Jagged2, Notch1, and Hey2 expression, and follicle formation in vitro. Maintaining high maternal P4 levels by daily injection also significantly suppressed the expression of Jagged2, Notch1, and Hey2, and follicle formation during late pregnancy. Based on immunohistochemistry, Jagged2 was localized in oocytes and Notch1 was strongly stained in pre-granulosa cells in 19.5 dpc ovaries. Suppression of their function by antibody addition and RNAi markedly inhibited nest breakdown and follicle formation. Taken together, these results demonstrate that maternal P4 levels during midpregnancy can inhibit the expression of Jagged2 and Notch1, which are involved in primordial folliculogenesis, in the mouse fetal ovary.