Inositol 1,4,5-trisphosphate signals the motile response of fish chromatophores. I, Aggregation of pigment in the tilapia melanophore
Inositol 1,4,5-trisphosphate signals the motile response of fish chromatophores. I, Aggregation of pigment in the tilapia melanophore
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肌醇 1,4,5-三磷酸发出鱼色素细胞运动反应的信号。
DOI：
10.1002/jez.1402590103
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发表时间：
1991
影响因子：
--
通讯作者：
N. Oshima
中科院分区：
文献类型：
--
作者：
R. Fujii;H. Wakatabi;N. Oshima
经表面活性剂brij58处理后，发现肌醇1,4,5-三磷酸（IP3）在罗非鱼（Oreochromis niloticus）原代培养的黑色素细胞内有效地引起黑素体聚集。1-单磷酸肌醇（IP）和1,4-二磷酸肌醇（IP2）没有这种作用。酚妥拉明（一种α -肾上腺素溶解剂）和吡唑嗪（一种更特异的α - 1阻滞剂）不干扰IP3的作用，尽管它们在对抗去甲肾上腺素作用下的色素聚集方面有效。因此，IP3可能作为一种细胞内生理介质，在色素聚集刺激的信号转导中起作用，包括至少α 1-肾上腺素能刺激，可能通过从储存它们的细胞内区室释放Ca2+来实现。
After permeabilization of the cell membrance by the treatment with a surfactant, Brij 58, inositol 1,4,5-trisphosphate (IP3) was found to be effective in causing aggregation of melanosomes within melanophores in primary cultures of the tilapia, Oreochromis niloticus. Inositol 1-monophosphate (IP) and 1,4-bisphosphate (IP2) did not have such an effect. Phentolamine, an alpha-adrenolytic agent, and prazosin, a more specific alpha1-blocker, did not interfere with the action of IP3, even though they were effective in antagonizing the aggregation of pigment in response to norepinephrine. It appears, therefore, that IP3 may function as an intracellular physiological mediator in the signal transduction of pigment-aggregating stimuli, including at least alpha1-adrenergic stimuli, possibly via the release of Ca2+ from the intracellular compartments in which they are stored.