Attachment of Entamoeba histolytica to glass in a defined maintenance medium: specific requirement for cysteine and ascorbic acid.
Attachment of Entamoeba histolytica to glass in a defined maintenance medium: specific requirement for cysteine and ascorbic acid.
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溶组织内阿米巴在规定的维持介质中附着在玻璃上：对半胱氨酸和抗坏血酸的特定要求。
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
Louis S. Diamond
中科院分区：
文献类型：
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作者：
Frances D. Gillin;Louis S. Diamond
溶组织内阿米巴滋养体在含有牛血清白蛋白和维生素溶液(维持介质1)的平衡盐溶液中，需要半胱氨酸和抗坏血酸，才能附着在玻璃上、伸长和阿米巴移动，以及短期(12-24小时)存活。如果半胱氨酸和抗坏血酸的唯一功能是降低氧化还原电位，其他还原剂应该是有效的。然而，在抗坏血酸存在的情况下对半胱氨酸的要求是高度特定的。D-半胱氨酸和L-半胱氨酸同样有效；然而，在所测试的许多其他化合物中，只有硫代乙醇酸、抗坏血酸或L-半胱氨酸(按降序)具有一定的活性。在氮气气氛下，仍然需要半胱氨酸和抗坏血酸，尽管它们的浓度可以减半。半胱氨酸-抗坏血酸剥夺5分钟后，阿米巴在维持培养液中的附着能力不可逆转地丧失；然而，将阿米巴转移到生长介质中30分钟内，阿米巴的存活率并没有下降。无论抗坏血酸浓度或阿米巴存在与否，半胱氨酸硫醇基团都被迅速氧化，但抗坏血酸延长了阿米巴的附着时间。
Cysteine and ascorbic acid were previously shown to be required by Entamoeba histolytica trophozoites for attachment to glass, elongation, and ameboid movement as well as for short-term (12-24 h) survival in a balanced salt solution containing bovine serum albumin and a vitamin solution (Maintenance Medium 1). If the only function of cysteine and ascorbate was to decrease the redox potential, other reducing agents should be effective. However, the requirement for cysteine in the presence of ascorbic acid was highly specific. Equally effective were D- and L-cysteine; however, of many other compounds tested, only thioglycolic acid, ascorbic acid, or L-cystine (in decreasing order) were somewhat active. Under N2 atmosphere, cysteine and ascorbic acid were still required, although their concentrations could be halved. The ability to attach in the maintenance medium was irreversibly lost after only 5 min of cysteine-ascorbic acid deprivation; however, there was no decrease in viability when the amebae were transferred to growth medium within 30 min. Cysteine thiol groups in the medium were oxidized rapidly regardless of the concentration of ascorbic acid or the presence of amebae; however, ascorbic acid prolonged attachment of amebae.