Expression of the metabotropic glutamate receptor mGluR1α and the ionotropic glutamate receptor GluR1 in the brain during the postnatal development of normal mouse and in the cerebellum from mutant mice
Expression of the metabotropic glutamate receptor mGluR1α and the ionotropic glutamate receptor GluR1 in the brain during the postnatal development of normal mouse and in the cerebellum from mutant mice
复制标题
代谢型谷氨酸受体 mGluR1α 和离子型谷氨酸受体 GluR1 在正常小鼠出生后发育过程中的大脑和突变小鼠小脑中的表达
DOI：
10.1002/jnr.490360104
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发表时间：
1993
影响因子：
4.2
通讯作者：
K. Mikoshiba
中科院分区：
文献类型：
--
作者：
Y. Ryo;Y. Ryo;A. Miyawaki;T. Furuichi;K. Mikoshiba
Expression of the metabotropic glutamate receptor type 1α (mGluR1α) and the non‐N‐methyl‐D‐aspartate (NMDA) ionotropic glutamate receptor type 1 (GluR1) in mouse brain was investigated using the antibodies raised against the synthetic peptides corresponding to their C‐terminal amino acid sequences. Both receptor proteins are glycosylated predominantly in an asparagine‐linked manner, and are abundant in post‐synaptic membranes. We showed that mGluR1α and GluR1 expression within the first 3 postnatal weeks undergoes dramatic changes in time and space, i.e., in the hippocampus and cerebellum. These spatio‐temporal expression patterns appear to be correlated with the postnatal ontogenesis and establishment of the glutamatergic neurotransmission system in the hippocampus and cerebellum, cell migration, dendritic and axonal growth, spine formation, and synaptogenesis. In the adult cerebellum, mGluR1α is intensely expressed in Purkinje neurons and GluR1 in Bergmann glial cells. Both receptors are expressed to a fair degree in weaver mutant cerebellum despite granule cell degeneration. However, the intrinsic expression levels of both mGluR1α and GluR1 are markedly reduced in the cerebellum of the Purkinje cell‐deficient and underdeveloped mutant mice, Purkinje‐cell‐degeneration, Lurcher, and staggerer, suggesting that GluR1 expression in Bergmann glia cells may be correlated with the sustained interaction with adjacent Purkinje neurons. © 1993 Wiley‐Liss, Inc.
