Theoretical and Practical Aspects of B‐Cell Activation: Murine and Human Systems
Theoretical and Practical Aspects of B‐Cell Activation: Murine and Human Systems
复制标题
B 细胞激活的理论和实践方面：小鼠和人类系统
作者：
R. Zubler;C. Werner‐Favre;L. Wen;Ken‐Ichi Sebcita;C. Straub
We have reviewed observations which were made during studies of murine and human B-cell responses in vitro. One currently faces difficulties in drawing any clear schema as to which external signals elicit which responses (activation, proliferation, differentiation) in B cells. However, the most potent antigen-dependent or polyclonal B-cell responses in vitro occur when, in addition to various cytokines, accessory cells, serum etc., the cultures contain either a) intact T-helper cells which enter into cell-to-cell contact with B cells, or b) some B-cell "mitogen" (T-independent antigen). Murine B cells activated with LPS and anti-Ig antibodies represent a model for the study of IL2 receptor expression and function. LPS does not act on human B cells. Certain mutant EL-4 thymoma cells are potent activators of murine and human B cells via a direct cell-to-cell interaction. The majority of human B cells can be induced to proliferate and generate a few hundred antibody-secreting cells each in the presence of such thymoma cells and a mixture of cytokines. From a practical point of view, this observation should be useful in a variety of investigations such as the analysis of the human B-cell specificity repertoire.
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抗原特异性 B 细胞和 T 细胞之间物理相互作用的表征。
DOI：
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发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Sanders,VM;Snyder,JM;Uhr,JW;Vitetta,ES
通讯作者：
Vitetta,ES
增强和抑制性 IgE 结合因子由单个克隆基因表达。
DOI：
10.1073/pnas.84.3.809
发表时间：
1987
影响因子：
11.1
作者：
Martens,CL;Jardieu,P;Trounstine,ML;Stuart,SG;Ishizaka,K;Moore,KW
通讯作者：
Moore,KW
单克隆抗体与 Epstein Barr 病毒 (EBV)/CR2 受体的结合可诱导人 B 淋巴细胞的激活和分化。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Nemerow,GR;McNaughton,ME;Cooper,NR
通讯作者：
Cooper,NR
分子量为 12,000 的人 B 细胞生长因子的细胞表面受体的表征。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Mehta,SR;Grant,SR;Maizel,AL
通讯作者：
Maizel,AL
抗 Ig 诱导肌醇 1,4,5-三磷酸的释放，介导 B 淋巴细胞中细胞内 Ca 储存的动员。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ransom,JT;Harris,LK;Cambier,JC
通讯作者：
Cambier,JC