Transgenic cotton resistant to herbicide bialaphos
Transgenic cotton resistant to herbicide bialaphos
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DOI：
10.1023/a:1018483300902
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发表时间：
1997-11-01
影响因子：
3
通讯作者：
John, ME
中科院分区：
文献类型：
--
作者：
Keller, G;Spatola, L;John, ME
双磷是一种非选择性除草剂，通过基因工程将其抗性引入棉花。利用粒子轰击的方法，将吸湿链霉菌（Streptomyces）的一个编码磷酸丙氨酸乙酰转移酶（phosphinothricin acetyltransferase, bar）的基因插入棉花优良品种中。基于标记基因-葡萄糖醛酸酶（gus）的表达，共获得18株Pima （Gossypium barbadense）、45株DP50 （G. hirsutum L.）、20株Coker 312 （G. hirsutum）和2株El Dorado （G. hirsutum）转基因植株。通过Southern blot分析证实了bar基因与棉花基因组DNA的整合，并通过northern blot和酶分析证实了该基因的表达。在温室试验中，除草剂（Basta(R)）耐受性高达15,000 ppm。新引入的除草剂耐受性性状在种系转化体的后代中以孟德尔方式遗传。这项研究表明，粒子轰击有可能将重要的商业基因直接引入棉花的优良品种。这种基因转移方式可以加速转基因棉花产品进入世界市场。
Resistance to bialaphos, a non-selective herbicide, was introduced into cotton through genetic engineering. A gene encoding phosphinothricin acetyltransferase (bar) from Streptomyces hygroscopicus was inserted into elite varieties of cotton through particle bombardment. Based on the marker gene, beta-glucuronidase ( gus) expression, a total of 18 Pima (Gossypium barbadense), 45 DP50 (G. hirsutum L.), 20 Coker 312 (G. hirsutum) and 2 El Dorado (G. hirsutum) transgenic plants were recovered. Integration of the bar gene into cotton genomic DNA was confirmed by Southern blot analysis and gene expression was confirmed by northern blot and enzyme assays. Herbicide (Basta(R)) tolerance up to 15,000 ppm was demonstrated in greenhouse trials. The newly introduced herbicide tolerance trait is inherited in a Mendelian fashion in the progenies of germline transformants. This study demonstrates the potential for particle bombardment to introduce commerically important genes directly into elite varieties of cotton. This mode of gene transfer can expedite the introduction of transgenic cotton products into world markets.