SEROTYPIC SIMILARITY AND DIVERSITY OF ROTAVIRUSES OF MAMMALIAN AND AVIAN ORIGIN AS STUDIED BY PLAQUE-REDUCTION NEUTRALIZATION
SEROTYPIC SIMILARITY AND DIVERSITY OF ROTAVIRUSES OF MAMMALIAN AND AVIAN ORIGIN AS STUDIED BY PLAQUE-REDUCTION NEUTRALIZATION
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DOI：
10.1093/infdis/149.5.694
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发表时间：
1984-01-01
影响因子：
6.4
通讯作者：
KAPIKIAN, AZ
中科院分区：
文献类型：
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作者：
HOSHINO, Y;WYATT, RG;KAPIKIAN, AZ
共检测了来自7种哺乳动物（人类和猪各4种，马和猿各2种，犬和牛各1种）和2种禽类（火鸡和鸡各1种）的16种不同轮状病毒毒株进行斑块减少中和试验。根据.gtoreq建立了7种抗原不同的血清型。同源和异源互惠中和抗体滴度相差20倍。血清型1 （Wa株）和血清型2 （DS-1株）仅从人体内恢复。血清型3包括人轮状病毒WALK 57/14株、恒河猴轮状病毒MMU18006株、长尾猴轮状病毒SA-11株、犬轮状病毒Cu-1株和马轮状病毒H-2株。在两人身上发现了新建立的血清4型病毒(圣托马斯菌株；猪（菌株Gottfried， SB-1A和SB-2）。猪（株OSU）和马（株H-1）轮状病毒构成可能的第5血清型。牛轮状病毒（NCDV株）为第6血清型，鸡轮状病毒（ch2株）为第7血清型，该病毒与火鸡轮状病毒（Ty 1株）有主株关系。从这项研究中出现了一个令人惊讶的观察结果，即存在一种轮状病毒（猪株SB-1A）桥接血清型4和5。
A total of 16 different strains of rotavirus derived from 7 mammalian species (4 each from human and porcine species, 2 each from equine and simian species and 1 each from canine and bovine species) and 2 avian species (1 each from turkeys and chickens) were examined in plaque-reduction neutralization tests. Seven antigenically distinct serotypes were established on the basis of a .gtoreq. 20-fold difference between titers of homologous and heterologous reciprocal neutralizing antibodies. Serotypes 1 (strain Wa) and 2 (strains DS-1) were recovered only from humans. Serotype 3 included human rotavirus strain WALK 57/14, rhesus monkey rotavirus strain MMU18006, vervet monkey rotavirus strain SA-11, dog rotavirus strain Cu-1 and horse rotavirus strain H-2. The newly established serotype 4 was identified in both humans (strain St. Thomas no. 4) and pigs (strains Gottfried, SB-1A, and SB-2). Porcine (strain OSU) and equine (strain H-1) rotavirus made up a possible-5th serotype. Bovine rotavirus (strain NCDV) constituted a 6th serotype and chicken rotavirus (strain Ch 2), which had a prime-strain relation with turkey rotavirus (strain Ty 1), was designated serotype 7. A surprising observation that emerged from this study was the existence of a rotavirus (porcine strain SB-1A) bridging serotypes 4 and 5.