Loss of heterozygosity of 14q32 in colorectal carcinoma
Loss of heterozygosity of 14q32 in colorectal carcinoma
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DOI：
10.1016/s0165-4608(98)00242-8
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发表时间：
1999-06-01
影响因子：
--
通讯作者：
Isobe, M
中科院分区：
文献类型：
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作者：
Bando, T;Kato, Y;Isobe, M
以往的等位基因分型研究表明，染色体14 q上的杂合性丢失（洛）可能是这种肿瘤类型中常见的遗传改变。本研究的目的是确定大肠癌14 q32区域洛的精确频率，并确定最小的洛区域。应用聚合酶链反应（PCR）技术，对66例原发性结直肠癌组织14 q32的6个高度多态性微卫星位点进行缺失定位，分析其洛缺失（LOH）情况。在所有分析的结直肠癌病例中，平均信息率为68.2%。在有信息的病例中，14 q32位点的平均洛缺失率为44.1%，其中D14 S267位点的杂合性缺失频率最高，为50.0%。洛缺失的最小区域为14 q32的D14 S65和D14 S250。本研究为从该区域分离肿瘤抑制基因提供了有用的信息。(C)Elsevier Science Inc.，1999. All rights reserved.
Previous allelotyping studies on colorectal carcinoma suggest that loss of heterozygosity (LOH) on chromosome 14q may be a common genetic alteration in this tumor type. The purpose of this study was to determine a precise frequency of LOH at 14q32 region in colorectal carcinomas and to define a minimal region of LOH. LOH at 14q32 in 66 primary colorectal carcinomas were analyzed by polymerase chain reaction-based deletion mapping using six highly polymorphic microsatellites. The average rate of informative data was 68.2% in all cases of colorectal carcinomas analyzed. The average rate of LOH at 14q32 was 44.1%, with the highest frequency of 50.0% at D14S267 locus in the informative cases. The minimal region of LOH was defined by markers D14S65 and D14S250 at 14q32. The present data provide useful information for the isolation of tumor suppressor genes from this region. (C) Elsevier Science Inc., 1999. All rights reserved.