In situ ligation: a decade and a half of experience.
In situ ligation: a decade and a half of experience.
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原位结扎：十五年的经验。
DOI：
10.1007/978-1-60327-409-8_5
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Didenko,VladimirV
中科院分区：
文献类型：
--
作者：
Hornsby,PeterJ;Didenko,VladimirV
原位连接（ISL）方法通过特征性DNA双链断裂的存在来检测凋亡细胞。将标记的双链探针连接至双链断裂的原位组织切片。与流行的TUNEL测定一样，ISL基于凋亡核酸酶对DNA的持续破坏来检测细胞凋亡。与TUNEL相比，它对细胞凋亡的特异性更强，而对其他DNA损伤原因（可修复损伤和坏死）的特异性更强。在其推出以来的15年中，ISL已被用于数百种出版物。在这里，我们回顾了该方法的发展，其现状，其用途和局限性。
Thein situligation (ISL) methodology detects apoptotic cells by the presence of characteristic DNA double-strand breaks. A labeled double-stranded probe is ligated to the double-strand breaksin situon tissue sections. Like the popular TUNEL assay, ISL detects cells in apoptosis based on the ongoing destruction of DNA by apoptotic nucleases. In comparison to TUNEL, it is more specific for apoptosis versus other causes of DNA damage, both repairable damage and necrosis. In the decade and a half since its introduction, ISL has been used in several hundred publications. Here we review the development of the method, its current status, and its uses and limitations.