CCR6hiCD11cint B cells promote M-cell differentiation in Peyer's patch
CCR6hiCD11cint B cells promote M-cell differentiation in Peyer's patch
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DOI：
10.1093/intimm/dxq478
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发表时间：
2011-04-01
影响因子：
4.4
通讯作者：
Ohno, Hiroshi
中科院分区：
文献类型：
--
作者：
Ebisawa, Masashi;Hase, Koji;Ohno, Hiroshi
M细胞负责Peyer集合淋巴结（PP）中粘膜抗原的摄取。M细胞的分化被认为是由卵泡相关上皮细胞和PP细胞之间的相互作用诱导的;然而，什么类型的免疫细胞起M细胞诱导剂的作用仍然是难以捉摸的。在这里，我们试图确定的细胞，作为一个M-细胞诱导PP。我们发现，一个独特的B细胞亚群的特点是CCR 6（hi）CD 11 c（int）居住在上皮下圆顶（SED）在小鼠PP。CCR 6（hi）CD 11 c（int）B细胞对CCL 20表现出趋化性迁移。此外，这种独特的B细胞亚群在CCR 6缺陷小鼠的PP中显著减少，表明CCR 6（hi）CD 11 c（int）B细胞的SED定位最有可能受到CCL 20-CCR 6系统的调节。同时，CCR 6缺陷导致M细胞显著减少。此外，从野生型小鼠中过继转移CCR 6（hi）CD 11 c（int）B细胞恢复了CCR 6缺陷小鼠中M细胞的减少。总体而言，通过CCL 20-CCR 6系统对CCR 6（hi）CD 11 c（int）B细胞的空间调节可能在小鼠M细胞分化中起重要作用。
M cells are responsible for uptake of mucosal antigens in Peyer's patches (PPs). Differentiation of M cells is thought to be induced by interactions between follicle-associated epithelium and PP cells; however, it remains elusive what types of immune cells function as M-cell inducers. Here, we attempted to identify the cells that serve as an M-cell inducer in PP. We found that a unique B-cell subset characterized by CCR6(hi)CD11c(int) resided in the subepithelial dome (SED) in mouse PP. CCR6(hi)CD11c(int) B cells showed chemotactic migration in response to CCL20. Furthermore, this unique B-cell subset substantially decreased in PP of CCR6-deficient mice, indicating that the SED localization of CCR6(hi)CD11c(int) B cells is most likely regulated by the CCL20-CCR6 system. Concomitantly, CCR6 deficiency caused remarkable decrement of M cells. Moreover, adoptive transfer of CCR6(hi)CD11c(int) B cells from wild-type mice restored the M-cell decrement in CCR6-deficient mice. Collectively, the spatial regulation of CCR6(hi)CD11c(int) B cells via the CCL20-CCR6 system may play a vital role in M-cell differentiation in mice.