Multiple functions of Drosophila heat shock transcription factor in vivo
Multiple functions of Drosophila heat shock transcription factor in vivo
复制标题
DOI：
10.1093/emboj/16.9.2452
复制
发表时间：
1997-05-01
期刊：
影响因子：
11.4
通讯作者：
Wu, C
中科院分区：
文献类型：
--
作者：
Jedlicka, P;Mortin, MA;Wu, C
热休克转录因子（HSF）是真核生物中热休克蛋白（hsp）基因的转录激活因子。为了阐明果蝇中HSF的生理功能，我们分离了HSF基因中的致死突变。使用一个条件等位基因，我们表明，HSF有一个重要的作用，在生物体的生存能力，极端的热应激。与以前用酵母HSF获得的结果相反，果蝇蛋白对于一般细胞生长或活力是不稳定的。然而，在正常生长条件下，卵子发生和早期幼虫发育需要它。果蝇HSF的这两种发育功能在遗传上是可分离的，并且似乎不通过HSPs的诱导来介导，这暗示了HSF的一种新的作用，该作用可能与其作为应激反应转录激活因子的特征功能无关。
Heat shock transcription factor (HSF) is a transcriptional activator of heat shock protein (hsp) genes in eukaryotes. In order to elucidate the physiological functions of HSF in Drosophila, we have isolated lethal mutations in the hsf gene. Using a conditional allele, we show that HSF has an essential role in the ability of the organism to survive extreme heat stress. In contrast to previous results obtained with yeast HSF, the Drosophila protein is dispensable for general cell growth or viability. However, it is required under normal growth conditions for oogenesis and early larval development. These two developmental functions of Drosophila HSF are genetically separable and appear not to be mediated through the induction of HSPs, implicating a novel action of HSF that may be unrelated to its characteristic function as a stress-responsive transcriptional activator.