Osteoclast precursor cells.
Osteoclast precursor cells.
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DOI：
10.1007/978-0-387-72009-8_10
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发表时间：
2007
影响因子：
--
通讯作者：
J. Lorenzo
中科院分区：
文献类型：
--
作者：
J. Lorenzo
The hematopoietic origin of the osteoclast is now clear. Walker (Walker 1975b, Walker 1975a, Walker 1975c) first demonstrated that the precursor cells of osteoclasts were hematopoietic. These studies showed that the transplant of spleen cells from osteopetrotic mice, which have dysfunctional osteoclasts, into irradiated normal animals caused the normal animals to become osteopetrotic within four weeks. Conversely, it was shown that transplant of normal spleen cells into irradiated osteopetrotic mice caused the osteopetrotic mice to develop normal bone remodeling within the same time period. More recently, bone marrow transplants into humans with osteopetrosis have, in selected cases, led to reversal of the condition (Coccia, Krivit, Cervenka, et al. 1980).Testa et al. first illustrated that feline bone marrow cultures could be induced to form multinucleated osteoclast-like cells (OCL) (Testa, Allen, Lajtha, et al. 1981, Allen, Testa, Suda, et al. 1981). Subsequently, Ibbotson et al characterized this system and demonstrated that stimulators of bone resorption enhanced the rate of formation of OCL (Ibbotson, Roodman, McManus, et al. 1984). Culture systems that use human or mouse marrow have been extensively studied and these demonstrate that the OCL that form have many characteristics of true osteoclasts. These include the abundant production of tartrate resistant acid phosphatase (TRAP) and calcitonin receptors (CTR) as well as the ability to form resorption lacunae when the cells are cultured on dentin or bone slices. Udagawa et al showed that spleen cells from mice could be cultured with stromal cell lines or primary osteoblastic cells and induced to form OCL (Udagawa, Takahashi, Akatsu, et al. 1989). The cells formed numerous resorption lacunae and had other characteristics of true osteoclasts. These authors also demonstrated that cell contact between hematopoietic osteoclast precursors in the spleen cell population and stromal or osteoblastic precursors was essential for osteoclast formation.
