Crucial role of thyroid hormone in x-ray-induced neoplastic transformation in cell culture.
Crucial role of thyroid hormone in x-ray-induced neoplastic transformation in cell culture.
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甲状腺激素在细胞培养中 X 射线诱导的肿瘤转化中的关键作用。
DOI：
10.1073/pnas.78.9.5708
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发表时间：
1981
影响因子：
11.1
通讯作者：
Edelman,IS
中科院分区：
文献类型：
--
作者：
Guernsey,DL;Borek,C;Edelman,IS
将小鼠胚胎成纤维细胞 (C3H/10T1/2) 在不含甲状腺激素的培养基中孵育 1 周，使细胞完全抵抗 X 射线剂量（4 格雷）的转化作用，在补充有三碘甲状腺原氨酸 (T3) (1 nM) 的培养基中产生约 10(-3) 的转化频率（每个存活细胞的病灶数）。关于添加或去除激素时间的研究表明，在照射前 12 小时添加 T3 时效果最大，并且从照射时间到病灶出现（6 周）的进展与激素的存在或不存在无关。 X 射线诱导的转化对培养基中 T3 浓度的依赖性实际上与 Na+,K+-ATP 酶活性增强的依赖性相同。后一种效应被用作 T3 诱导蛋白质合成的量度。蛋白质合成参与该过程的进一步迹象是放线菌酮在抑制 50% 蛋白质合成的浓度 (100 ng/ml) 时消除了 T3 和 X 射线依赖性转化。我们认为甲状腺激素诱导宿主蛋白的合成，该蛋白是 X 射线介导的转化的必然参与者。
Incubation of mouse embryo fibroblasts (C3H/10T1/2) in media depleted of thyroid hormone for 1 week rendered the cells completely resistant to the transforming action of an x-ray dose, 4 grays, that yields transformation frequencies (no. foci per surviving cells) of approximately 10(-3) in media supplemented with triiodothyronine (T3) (1 nM). Studies on the timing of the additions or removal of the hormone indicate that T3 was maximally effective when added 12 hr before irradiation and that progression from the time of irradiation to the appearance of foci (6 weeks) was independent of the presence or absence of the hormone. The dependence of x-ray-induced transformation on the concentration of T3 in the medium was virtually the same as that for augmentation of Na+,K+-ATPase activity. The latter effect was used as a measure of T3 induction of protein synthesis. A further indication of the involvement of protein synthesis in the process is the abolition of T3- and x-ray-dependent transformation by cycloheximide at a concentration (100 ng/ml) that inhibits 50% of protein synthesis. We propose that thyroid hormone induces the synthesis of a host protein that is an obligatory participant in x-ray-mediated transformation.