QUANTITATION OF RAT EMBRYONIC-DEVELOPMENT INVITRO - A MORPHOLOGICAL SCORING SYSTEM
QUANTITATION OF RAT EMBRYONIC-DEVELOPMENT INVITRO - A MORPHOLOGICAL SCORING SYSTEM
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DOI：
10.1002/tera.1420240108
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
FABRO, S
中科院分区：
文献类型：
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作者：
BROWN, NA;FABRO, S
为了实现全胚胎培养技术的最大潜力，必须开发明确的参数来定量估计胚胎生长和发育。设计了一个客观的评分系统，它提供了形态发育的精确测量。对怀孕第 10、11、12 和 13 天（精子阳性=第 1 天）来自母鼠的大鼠受孕进行检查，选择了 17 种形态特征用于系统。每个特征最多定义了 6 个发展阶段，并分配了 0-5 的分数。单个胚胎的得分总和被视为总体形态得分（MS）。该系统前瞻性地应用于 103 只 9-12.7 天的受孕 Sprague-Dawley 大鼠（0 = 交配时间）。评分随胚胎年龄（EA，天数）的变化呈高度线性：MS = -202.28 + 20.932 EA (R2 = 0.991)。因此，形态学评分可用于计算表观胚胎年龄；计算出的预测标准误差为.+-。 2.2小时在此期间，体节数量 (SN) 随年龄呈线性变化：SN = -126.23 + 13.217 EA (R2 = 0.982)，可用于估计发育。表观胚胎年龄预测的标准误差比形态学评分的标准误差大（±3.1 小时），这一单一特征不一定反映整体发育。检查了几个参数作为胚胎生长的估计，与发育不同。卵黄囊直径、冠臀长度和头部长度随着 EA 的变化呈二次函数变化。总胚胎蛋白随 EA 呈对数增加，被认为是胚胎生长的最合适的衡量标准。在胚胎培养实验中使用形态学评分系统提供了胚胎发育的精确指数，有助于检测特定原基的迟缓或畸形发生，并允许对发育和生长进行定量比较。
To realize the maximum potential of whole-embryo culture techniques, definitive parameters must be developed for the quantitative estimation of embryonic growth and development. An objective scoring system was devised which provides a precise measure of morphological development. Examination of rat conceptuses from dams on pregnancy days 10, 11, 12 and 13 (sperm positive = day 1) led to the selection of 17 morphological features for use in the system. Up to 6 developmental stages of each feature were defined and assigned scores of 0-5. The numerical total of scores for an individual embryo is taken as the overall morphological score (MS). The system was applied prospectively to 103 Sprague-Dawley rat conceptuses aged 9-12.7 days (0 = time of copulation). The variation of score with embryonic age (EA, days) was highly linear: MS = -202.28 + 20.932 EA (R2 = 0.991). Thus, the morphological score can be used to compute apparent embryonic age; the calculated standard error of prediction is .+-. 2.2 h. The number of somites (SN) varied linearly with age over this period: SN = -126.23 + 13.217 EA (R2 = 0.982), and could be used to estimate development. Standard error for prediction of apparent embryonic age is greater (.+-. 3.1 h) than that for morphological score, this single feature will not necessarily reflect overall development. Several parameters were examined as estimates of embryonic growth, as distinct from development. Yolk sac diameter, crown-rump length and head length varied with EA as quadratic functions over this time. Total embryonic protein increased logarithmically with EA and was considered to be the most suitable measure of embryonic growth. Use of the morphological scoring system in embryo culture experiments provides a precise index of embryonic development, aids the detection of retardation or dysmorphogenesis of specific primordia and allows a quantitative comparison of development and growth.