ANTIBODIES SPECIFIC FOR LEFT-HANDED Z-DNA
ANTIBODIES SPECIFIC FOR LEFT-HANDED Z-DNA
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DOI：
10.1073/pnas.78.6.3546
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
RICH, A
中科院分区：
文献类型：
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作者：
LAFER, EM;MOLLER, A;RICH, A
溴化聚（dG-dC）.制备了在生理盐条件下形成稳定Z-DNA螺旋的poly（dG-dC）。用溴化和未溴化的聚（dG-dC）免疫兔和小鼠。与甲基化牛血清白蛋白复合的聚（dG-dC）。产生了对Z-DNA具有特异性的抗体。在用低盐稳定的Z-DNA [Br-聚（dG-dC）.聚（dG-dC）]和用未溴化的B-DNA形式的聚合物免疫的动物的血清中。未溴化的聚（dG-dC）. poly（dG-dC）可能通过与碱性蛋白甲基化牛血清白蛋白结合而部分转化为Z-DNA。除特异性抗Z-DNA抗体群外，还发现了2种其它感兴趣的抗体群类型，其中一种与Z和B形式的聚（dG-dC）均反应。聚（dG-dC）。这种抗体可以将聚合物从B-DNA转化为Z-DNA形式。另一种类型的抗体对聚（dG-dC）的B形式具有特异性。聚（dG-dC），并且完全不与Z形式反应。针对Z-DNA产生的抗体对Z-DNA具有高度特异性，并且不与B-DNA、RNA、DNA反应。RNA杂合体或许多其他多核苷酸。这种对Z-DNA的特异性将使它们有可能用作测定生物系统中Z-DNA存在的试剂。自身免疫性狼疮小鼠血清中有相当数量的天然抗Z-DNA抗体活性。
A brominated poly(dG-dC) .cntdot. poly(dG-dC) which forms a stable Z-DNA helix under physiological salt conditions was prepared. Rabbits and mice were immunized with brominated and unbrominated poly(dG-dC) .cntdot. poly(dG-dC) complexed with methylated bovine serum albumin. Antibodies specific for Z-DNA were produced. These antibodies were found in the sera of animals immunized with the low-salt stabilized Z-DNA [Br-poly(dG-dC) .cntdot. poly(dG-dC)] and in sera from animals immunized with the unbrominated B-DNA form of the polymer. The unbrominated poly(dG-dC) .cntdot. poly(dG-dC) was probably partially converted to Z-DNA by its combination with the basic protein methylated bovine serum albumin. In addition to specific anti-Z-DNA antibody populations, 2 other interesting types of antibody populations were found. One of these reacted with both the Z and B forms of poly(dG-dC) .cntdot. poly(dG-dC). This antibody may be converting the polymer from the B-DNA to the Z-DNA form. The other type of antibody was specific for a B form of poly(dG-dC) .cntdot. poly(dG-dC) and did not react at all with the Z form. The antibodies raised to Z-DNA were highly specific for Z-DNA and did not react with B-DNA, RNA, DNA .cntdot. RNA hybrids, or a number of other polynucleotides. This specificity for Z-DNA will make possible their use as reagents for determining the presence of Z-DNA in biological systems. Sera of autoimmune lupus mice had considerable amount of naturally occurring anti-Z-DNA antibody activity.