Defensin pattern in chronic gastritis: HBD-2 is differentially expressed with respect to Helicobacter pylori status
Defensin pattern in chronic gastritis: HBD-2 is differentially expressed with respect to Helicobacter pylori status
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慢性胃炎中的防御素模式：HBD-2 在幽门螺杆菌状态方面存在差异表达
DOI：
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发表时间：
2003
影响因子：
3.4
通讯作者：
K. Fellermann
中科院分区：
文献类型：
--
作者：
J. Wehkamp;K. Schmidt;K. Herrlinger;S. Baxmann;S. Behling;C. Wohlschläger;A. Feller;E. Stange;K. Fellermann
Background/Aims: Recent reports have suggested that Helicobacter pylori infection induces the mucosal antibiotic peptide human β defensin 2 (HBD-2). Therefore, the present study investigated mRNA and peptide expression of four different defensins in the upper gastrointestinal tract in patients with H pylori positive and negative chronic gastritis. Materials/Methods: Biopsies from the oesophagus to the duodenum were taken during routine gastroscopy in 71 individuals. Total RNA was extracted and the reverse transcription polymerase chain reaction was performed with primers for human defensins 5 and 6 (HD-5/6) or HBD-1 and HBD-2. Paraffin wax embedded tissue from gastric resections was tested for HD-5, HBD-1, and HBD-2 by immunohistochemistry. Results:Helicobacter pylori colonisation was associated with an increased percentage of positive biopsies with respect to HBD-2 in the corpus (p < 0.05). Helicobacter pylori had no impact on the gastric expression of HD-5 and HBD-1, whereas HD-6 was increased in the fundus. The abundant expression of α defensins in the duodenum and β defensins in the oesophagus served as a positive control in each individual. Immunohistochemical analysis confirmed the presence of the HD-5, HBD-1, and HBD-2 peptides in gastric resection specimens. Conclusions: The recently described induction of HBD-2 upon H pylori infection was confirmed in a clinical setting of chronic gastritis. This phenomenon may be mediated by components of the pathogen itself or may occur secondary to immune events in chronic inflammation.
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影响因子：
15.9
作者：
LEHRER, RI;BARTON, A;SELSTED, ME
通讯作者：
SELSTED, ME
影响因子：
29.4
作者：
Keates, S;Hitti, YS;Kelly, CP
通讯作者：
Kelly, CP
影响因子：
29.4
作者：
Fu, SD;Ramanujam, KS;Wilson, KT
通讯作者：
Wilson, KT
幽门螺杆菌在 cag 致病岛上的人胃细胞系 MKN45 细胞中诱导人 β-防御素-2 mRNA 表达。
影响因子：
3.1
作者：
Wada,A;Mori,N;Oishi,K;Hojo,H;Nakahara,Y;Hamanaka,Y;Nagashima,M;Sekine,I;Ogushi,K;Niidome,T;Nagatake,T;Moss,J;Hirayama,T
通讯作者：
Hirayama,T
影响因子：
4.4
作者：
Deborah O’Neil;E. Porter;D. Elewaut;G. Anderson;L. Eckmann;T. Ganz;M. Kagnoff
通讯作者：
Deborah O’Neil;E. Porter;D. Elewaut;G. Anderson;L. Eckmann;T. Ganz;M. Kagnoff