Experimental mouse ocular hypertension: Establishment of the model
Experimental mouse ocular hypertension: Establishment of the model
复制标题
DOI：
10.1167/iovs.03-0137
复制
发表时间：
2003-10-01
影响因子：
4.4
通讯作者：
Weinreb, RN
中科院分区：
文献类型：
--
作者：
Aihara, M;Lindsey, JD;Weinreb, RN
目的。目的：建立小鼠高眼压模型。方法：选用22只瑞士黑鼠。麻醉和散瞳后，通过吸出房水使前房变平。然后在角膜缘进行激光光凝(波长532 nm，功率200 mW，持续时间0.05秒，光斑尺寸200微米)。每周测量眼压(IOP)。用微针法治疗4周，每2周1次，共12周。在整个过程中进行裂隙灯生物显微镜检查，并对结构变化进行组织学评估。如果在前4周收集的眼压测量值的平均值增加30%或更多，或者在12周研究期间收集的所有测量值的平均值增加30%或更多，则认为治疗反应是成功的。结果：激光治疗眼在1-6周表现出明显高于对照眼的眼压(P<；0.001)。治疗后第1、4、12周的平均眼压明显高于对照眼(P<；0.001)。眼压分别升高7.1和3.8毫米汞柱。在治疗的前4周，%(14/22)的患者眼压持续升高。在整个12周的研究中，37%(7/19)的接受治疗的眼睛成功地保持了升高的眼压。6周后，高眼压往往恢复正常或几个汞低于正常。在12周的研究结束时，组织学分析显示眼前段没有炎性细胞，并证实激光光凝治疗闭合角。结论：该方法可使小鼠眼压持续升高，有望成为青光眼性视神经病变生物学机制研究的一种实验模型。
PURPOSE. To establish an experimental model of ocular hypertension in the mouse.METHODS. Twenty-two black Swiss mice were used. After anesthesia and pupil dilation, the anterior chamber was flattened by the aspiration of aqueous humor. Laser photocoagulation (532-nm wavelength, 200-mW power, 0.05-second duration, 200-mum spot size) then was performed at the limbus. Intraocular pressure (IOP) was measured weekly. for 4 weeks and biweekly for 12 weeks, by a microneedle method. Slit lamp biomicroscopy was performed throughout the period and the structural changes were assessed histologically. A treatment response was considered to be a success if either the mean of IOP measurements collected during the first 4 weeks was increased by 30% or more, or the mean of all measurements collected during the 12 week study period was increased by 30% or more.RESULTS. Laser-treated eyes showed significantly higher IOP than control eyes from 1 to 6 weeks (P < 0.001). The average IOP in treated eyes during the first,4 and 12 weeks was significantly higher than the control IOP (P < 0.001). These IOP increases were 7.1 and 3.8 mm Hg, respectively. During the first 4 weeks, sustained elevation of IOP was obtained in 64% (14/22) of the treated eyes. During the entire 12-week study, increased IOP was successfully maintained in 37% (7/19) of the treated eyes. After 6 weeks, elevated IOP often returned to normal or several turn Hg below normal. Histologic analysis at the end of the 12-week study showed no inflammatory cells in the anterior segment and confirmed that the angle was closed by the laser photocoagulation treatment.CONCLUSIONS. This method produces persistent IOP elevation in mouse eyes and may be a promising experimental model for the investigation of the biological mechanisms of glaucomatous optic neuropathy.