Gene expression of (pro)renin receptor is upregulated in hearts and kidneys of rats with congestive heart failure
Gene expression of (pro)renin receptor is upregulated in hearts and kidneys of rats with congestive heart failure
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DOI：
10.1016/j.peptides.2009.09.015
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发表时间：
2009-12-01
期刊：
影响因子：
3
通讯作者：
Imai, Yutaka
中科院分区：
文献类型：
--
作者：
Hirose, Takuo;Mori, Nobuyoshi;Imai, Yutaka
最近的研究表明，（前）肾素受体（（P）RR），一个新发现的成员，肾素-血管紧张素系统，与肾脏器官损伤。然而，心脏中的（P）RR的信息很少。为探讨（P）RR在心力衰竭中的调节作用，采用定量RT-PCR和免疫组织化学方法检测了（P）RR在冠状动脉结扎致充血性心力衰竭（CHF）大鼠心脏和肾脏中的表达。CHF大鼠心房、右心室、左心室非梗死区、左心室梗死区和肾脏中（P）RR mRNA的表达水平分别为假手术组的1.6倍、1.4倍、1.6倍、1.7倍和1.5倍。在CHF大鼠中，这些心和肾组织中编码肾素和血管紧张素原的mRNA的表达水平也增加。免疫组化显示心肌、肾小管细胞、心、肾血管平滑肌和内皮细胞均呈RR阳性（P <0.05）。CHF大鼠肾小管上皮细胞免疫组化染色较假手术组明显增强。这些结果表明，（P）RR在心力衰竭大鼠的心脏和肾脏中表达增加，并且（P）RR可能参与心力衰竭。(C)2009 Elsevier Inc. All rights reserved.
Recent studies have revealed that (pro)renin receptor ((P)RR), a newly identified member of the renin-angiotensin system, was associated with organ damage in the kidney. However, there has been little information for (P)RR in hearts. To investigate the regulation of (P)RR in heart failure, we examined the expression of (P)RR in hearts and kidneys of rats with congestive heart failure (CHF) due to coronary ligation by quantitative RT-PCR and immunohistochemistry. Significantly increased levels of (P)RR mRNA were found in the atrium, right ventricle, non-infarcted part of left ventricle, infarcted part of left ventricle and kidney of CHF rats, when compared with sham operated rats (about 1.6-fold, 1.4-fold, 1.6-fold, 1.7-fold and 1.5-fold, respectively). Expression levels of mRNAs encoding renin and angiotensinogen in these heart and kidney tissues were also increased in the CHF rats. Immunohistochemistry showed positive (P)RR immunostaining in the myocardium, the renal tubular cells, and vascular smooth muscle and endothelial cells in the heart and the kidney. The renal tubular cells were more intensely immunostained in CHF rats than in sham operated rats. These findings suggest that the expression of (P)RR is increased in the hearts and kidneys of rats with heart failure, and that (P)RR may contribute to heart failure. (C) 2009 Elsevier Inc. All rights reserved.