K+ channel mechanisms underlying cholinergic cutaneous vasodilation and sweating in young humans: roles of KCa, KATP, and KV channels?
K+ channel mechanisms underlying cholinergic cutaneous vasodilation and sweating in young humans: roles of KCa, KATP, and KV channels?
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年轻人胆碱能皮肤血管舒张和出汗的 K 通道机制：KCa、KATP 和 KV 通道的作用？
DOI：
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
G. Kenny
中科院分区：
文献类型：
--
作者：
Naoto Fujii;Jeffrey C. Louie;Brendan D. McNeely;Sarah Y. Zhang;M. Tran;G. Kenny
从胆碱能神经释放的乙酰胆碱参与皮肤血管舒张和出汗的热损失反应。钾离子通道被认为在调节胆碱能皮肤血管舒张和出汗中起作用，但哪些钾离子通道参与其调节仍不清楚。我们评估了以下假设：1）Ca（2+）激活的K（+）（KCa）、ATP敏感的K（+）（KATP）和电压门控的K（+）（KV）通道都有助于胆碱能皮肤血管舒张; 2）KV通道，而不是KCa和KATP通道，有助于胆碱能出汗。在13名年轻成人（24 ± 5岁）中，在皮内微透析部位评价了皮肤血管传导性（CVC）和出汗率，这些部位连续灌注：1）乳酸林格氏液（对照）、2）50 mM四乙基铵（KCa通道阻滞剂）、3）5 mM格列本脲（KATP通道阻滞剂）和4）10 mM 4-氨基吡啶（KV通道阻滞剂）。在所有部位，胆碱能皮肤血管舒张和出汗诱导乙酰甲胆碱（0.0125，0.25，5，100，和2,000 mM，各25分钟）的共同管理。乙酰甲胆碱诱导的CVC增加在KCa通道阻断剂的情况下相对于对照为0.0125（1 ± 1 vs. 9 ± 6%最大值）和5（2 ± 5 vs. 17 ± 14%max）mM乙酰甲胆碱，而在KATP（69 ± 7%max）和KV（57 ± 14%max）通道阻断剂存在下，与对照组（79 ± 6%max）相比，在100 mM乙酰甲胆碱下，其较低。此外，在2,000 mM乙酰甲胆碱时，KV通道阻滞剂部位乙酰甲胆碱诱导的出汗（0.42 ± 0.17 mg·min（-1）·cm（-2））低于对照组（0.58 ± 0.15 mg·min（-1）·cm（-2））。总之，我们发现，钾钙，KATP和KV通道发挥作用，胆碱能皮肤血管舒张，而只有KV通道有助于胆碱能出汗在常温下休息的人。
Acetylcholine released from cholinergic nerves is involved in heat loss responses of cutaneous vasodilation and sweating. K(+) channels are thought to play a role in regulating cholinergic cutaneous vasodilation and sweating, though which K(+) channels are involved in their regulation remains unclear. We evaluated the hypotheses that 1) Ca(2+)-activated K(+) (KCa), ATP-sensitive K(+) (KATP), and voltage-gated K(+) (KV) channels all contribute to cholinergic cutaneous vasodilation; and 2) KV channels, but not KCa and KATP channels, contribute to cholinergic sweating. In 13 young adults (24 ± 5 years), cutaneous vascular conductance (CVC) and sweat rate were evaluated at intradermal microdialysis sites that were continuously perfused with: 1) lactated Ringer (Control), 2) 50 mM tetraethylammonium (KCa channel blocker), 3) 5 mM glybenclamide (KATP channel blocker), and 4) 10 mM 4-aminopyridine (KV channel blocker). At all sites, cholinergic cutaneous vasodilation and sweating were induced by coadministration of methacholine (0.0125, 0.25, 5, 100, and 2,000 mM, each for 25 min). The methacholine-induced increase in CVC was lower with the KCa channel blocker relative to Control at 0.0125 (1 ± 1 vs. 9 ± 6%max) and 5 (2 ± 5 vs. 17 ± 14%max) mM methacholine, whereas it was lower in the presence of KATP (69 ± 7%max) and KV (57 ± 14%max) channel blocker compared with Control (79 ± 6%max) at 100 mM methacholine. Furthermore, methacholine-induced sweating was lower at the KV channel blocker site (0.42 ± 0.17 mg·min(-1)·cm(-2)) compared with Control (0.58 ± 0.15 mg·min(-1)·cm(-2)) at 2,000 mM methacholine. In conclusion, we show that KCa, KATP, and KV channels play a role in cholinergic cutaneous vasodilation, whereas only KV channels contribute to cholinergic sweating in normothermic resting humans.
钙激活钾通道在人体皮肤主动血管舒张中没有独立的作用，而是相互作用的。
DOI：
10.1152/japplphysiol.00358.2013
发表时间：
2013
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Brunt,ViennaE;Fujii,Naoto;Minson,ChristopherT
通讯作者：
Minson,ChristopherT
年轻人和老年人皮内 NO 给药期间的皮肤血流量：钙激活钾通道和环氧合酶的作用？
DOI：
10.1152/ajpregu.00041.2016
发表时间：
2016
期刊：
American journal of physiology. Regulatory, integrative and comparative physiology
影响因子：
--
作者：
Fujii,Naoto;Meade,RobertD;Minson,ChristopherT;Brunt,ViennaE;Boulay,Pierre;Sigal,RonaldJ;Kenny,GlenP
通讯作者：
Kenny,GlenP
影响因子：
3.3
作者：
Lee, K;Mack, GW
通讯作者：
Mack, GW