脳生切片へのコリンの取り込み及びアセチルコリンの放出に対する無酸素処理の影響: [11C] コリン-バイオラジオグラフィー法を用いた研究
脳生切片へのコリンの取り込み及びアセチルコリンの放出に対する無酸素処理の影響: [11C] コリン-バイオラジオグラフィー法を用いた研究
复制标题
缺氧处理对原始脑切片中胆碱摄取和乙酰胆碱释放的影响：使用[11C]胆碱生物放射成像方法的研究
DOI：
10.3769/radioisotopes.52.677
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
一典 寺崎
中科院分区：
文献类型：
--
作者：
徹 佐々木;善仁 船木;正典 小豆島;一典 寺崎
The uptake of choline for the synthesis and release of acetylcholine and the metabolism of glucose under anoxic conditions was investigated in brain slices by bioradiography using [N-methyl-11C] choline ( [11C] choline) and [18F] 2-fluoro-2-deoxy-D-glucose ( [18F] FDG ) . [11C] Choline uptake and the release of accumulated11Cradioactivity in brain slices decreased with anoxic treatment, whereas [18F] FDG uptake increased. The decrease of [11C] choline uptake and the11Cradioactivity accumulated in striatal slices were recovered by acetyl-L-carnitine, an acetyl-donor. However, this effect was not seen in cerebral cortex. These results indicate that choline uptake for the synthesis and release of acetylcholine in brain are energy sensitive. The cholinergic dysfunction in ischemic brain might be improved by compensating for energy loss.
