Differential Motion Dynamics of Synaptic Vesicles Undergoing Spontaneous and Activity-Evoked Endocytosis
Differential Motion Dynamics of Synaptic Vesicles Undergoing Spontaneous and Activity-Evoked Endocytosis
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DOI：
10.1016/j.neuron.2012.01.023
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发表时间：
2012-03-22
期刊：
影响因子：
16.2
通讯作者：
Klyachko, Vitaly A.
中科院分区：
文献类型：
--
作者：
Peng, Amy;Rotman, Ziv;Klyachko, Vitaly A.
突触囊泡的胞吞和胞吐通常由神经元活动驱动，但也可以自发发生。支持诱发和自发神经传递的囊泡之间的身份和差异仍然存在很大争议。在这里，我们结合了纳米分辨率成像与瞬态运动分析方法，以检查在生理条件下的海马末梢的单个突触囊泡的动态。我们发现，经历自发内吞和刺激内吞的囊泡在动态行为上有所不同，特别是参与定向运动的能力。我们的数据表明，这种运动差异取决于肌球蛋白家族的马达蛋白，特别是肌球蛋白II。在肌球蛋白II抑制剂的存在下，突触传递的分析证实了肌球蛋白II在诱发的，但不是自发的，神经传递中的特定作用，并建议肌球蛋白II介导的囊泡运动在支持囊泡动员在神经活动过程中的功能作用。
Synaptic vesicle exo- and endocytosis are usually driven by neuronal activity but can also occur spontaneously. The identity and differences between vesicles supporting evoked and spontaneous neuro-transmission remain highly debated. Here we combined nanometer-resolution imaging with a transient motion analysis approach to examine the dynamics of individual synaptic vesicles in hippocampal terminals under physiological conditions. We found that vesicles undergoing spontaneous and stimulated endocytosis differ in their dynamic behavior, particularly in the ability to engage in directed motion. Our data indicate that such motional differences depend on the myosin family of motor proteins, particularly myosin II. Analysis of synaptic transmission in the presence of myosin II inhibitor confirmed a specific role for myosin II in evoked, but not spontaneous, neurotransmission and also suggested a functional role of myosin II-mediated vesicle motion in supporting vesicle mobilization during neural activity.