Effects of functionalized and nonfunctionalized single-walled carbon nanotubes on root elongation of select crop species
Effects of functionalized and nonfunctionalized single-walled carbon nanotubes on root elongation of select crop species
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DOI：
10.1897/08-117.1
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发表时间：
2008-09-01
影响因子：
4.1
通讯作者：
Olszyk, David
中科院分区：
文献类型：
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作者：
Canas, Jaclyn E.;Long, Monique;Olszyk, David
单壁碳纳米管有许多潜在的有益用途，更多的应用正在不断被研究。然而，它们的独特性质引发了人们对毒性的潜在担忧，不仅对人类和动物，而且对植物也是如此。为了帮助制定确定纳米管对植物影响的方案，我们对植物毒性测试中常用的六种作物(甘蓝、胡萝卜、黄瓜、生菜、洋葱和番茄)进行了功能化和非功能化单壁碳纳米管对根伸长的影响的初步研究。用聚3-氨基苯磺酸对纳米管进行官能化处理。在暴露后0、24和48h测量根的生长。扫描电子显微镜用来评估碳纳米管的潜在吸收，并观察碳纳米管与根表面的相互作用。一般来说，非功能化碳纳米管比功能化碳纳米管对根长的影响更大。非功能化纳米管抑制了番茄的根伸长，促进了洋葱和黄瓜的根伸长。功能化纳米管抑制生菜根部伸长。卷心菜和胡萝卜不受任何一种形式的纳米管的影响。暴露于碳纳米管后的影响在24小时比48小时更明显。显微镜图像显示在根部表面存在纳米管片，但没有观察到明显的纳米管摄取。
Single-walled carbon nanotubes have many potential beneficial uses, with additional applications constantly being investigated. Their unique properties, however, create a potential concern regarding toxicity, not only in humans and animals but also in plants. To help develop protocols to determine the effects of nanotubes on plants, we conducted a pilot study on the effects of functionalized and nonfunctionalized single-walled carbon nanotubes on root elongation of six crop species (cabbage, carrot, cucumber, lettuce, onion, and tomato) routinely used in phytotoxicity testing. Nanotubes were functionalized with poly-3-aminobenzenesulfonic acid. Root growth was measured at 0, 24, and 48 h following exposure. Scanning-electron microscopy was used to evaluate potential uptake of carbon nanotubes and to observe the interaction of nanotubes with the root surface. In general, nonfunctionalized carbon nanotubes affected root length more than functionalized nanotubes. Nonfunctionalized nanotubes inhibited root elongation in tomato and enhanced root elongation in onion and cucumber. Functionalized nanotubes inhibited root elongation in lettuce. Cabbage and carrots were not affected by either form of nanotubes. Effects observed following exposure to carbon nanotubes tended to be more pronounced at 24 h than at 48 h. Microscopy images showed the presence of nanotube sheets on the root surfaces, but no visible uptake of nanotubes was observed.