The effects of intracortical endothelin-1 injections on skilled forelimb use: implications for modelling recovery of function after stroke
The effects of intracortical endothelin-1 injections on skilled forelimb use: implications for modelling recovery of function after stroke
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DOI：
10.1016/j.bbr.2003.07.006
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发表时间：
2004-04-02
影响因子：
2.7
通讯作者：
Bannerman, DM
中科院分区：
文献类型：
--
作者：
Gilmour, G;Iverson, SD;Bannerman, DM
不同的脑损伤诱导方法可导致不同的神经病理学后遗症。这可能是建立中风后功能恢复的动物模型的结果，因为实验性病变病理学进展的差异可能对用任何特定病变方法观察到的功能恢复的幅度和速率产生影响。在本研究中，一种新的方法产生局灶性缺血性病变皮层内微量注射内皮素-1（ET-1）进行了比较兴奋性毒性（微量注射喹啉酸）和机械（抽吸）病变程序。病变是单侧的，目标是在前肢代表区的感觉运动皮层。结果发现，所有三种类型的病变具有基本相同的效果，达到准确性在一个爪子达到任务，所有病变的动物显示出类似的，显着的长期缺陷，达到准确性和有限程度的恢复相对于假手术动物。损伤后第9周期间动物表现的离线分析表明，每个损伤组中的动物也显示出类似的缺陷。目前的结果表明，自发行为的后果，在大鼠的FL单侧病变似乎是独立的病变生产的性质。然而，增加的ET-1诱导的病变的表面有效性，加上易于控制病变的位置和程度提供了这种技术，使一个潜在的重要的动物模型研究药物对功能恢复中风后。(C)2003 Elsevier B. V.保留所有权利。
Different methods of inducing experimental brain lesions can result in distinct neuropathological sequelae. This could be of consequence ill attempts to establish animal models of recovery of function following stroke, as differences in the progression of experimental lesion pathology may have an impact on the magnitude and rate of recovery of function observable with any particular lesioning method. In the present study, a novel method of producing a focal ischaemic lesion by intracortical microinjection of endothelin-1 (ET-1) was compared with excitotoxic (microinjection of quinolinic acid) and mechanical (aspiration) lesioning procedures. Lesions were unilateral and were targeted at the forelimb representation zone in sensorimotor cortex. It was found that all three types of lesion had an essentially identical effect with regard to reaching accuracy in a paw-reaching task, All lesioned animals displayed a similar, significant long-term deficit in reaching accuracy and limited degree of recovery relative to sham animals. Off-line analysis of the performance of animals during post-lesion week 9 indicated that animals in each lesion group also displayed a similar deficit. The current results suggest that the spontaneous behavioural consequences of a unilateral lesion of FL in the rat appear to be independent of the nature of lesion production. However, the increased face validity of an ET-1-induced lesion, coupled with the ease of control of lesion placement and extent offered by this technique make for a potentially important animal model for research into drug effects on recovery of function following stroke. (C) 2003 Elsevier B.V. All rights reserved.