Role of the interferons in CD64 and CD169 expressions in whole blood: Relevance in the balance between viral- or bacterial-oriented immune responses
Role of the interferons in CD64 and CD169 expressions in whole blood: Relevance in the balance between viral- or bacterial-oriented immune responses
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DOI：
10.1002/iid3.289
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发表时间：
2020-02-07
影响因子：
3.2
通讯作者：
Malergue, Fabrice
中科院分区：
文献类型：
--
作者：
Bourgoin, Penelope;Biechele, Geraldine;Malergue, Fabrice
在细菌感染期间，中性粒细胞上的CD 64表达增加。最近已经发现在病毒感染期间单核细胞上的CD 169表达增加。通常，干扰素α（IFN α）和IFN γ是感染性宿主免疫应答的关键驱动因素。本研究的目的是探讨如果这些干扰素和两个biomarkers.Methods之间存在联系，从健康志愿者的全血样本进行了刺激与干扰素，白细胞介素，或感染性提取物，模仿感染状态。CD 64和CD 169的表达在这些样品中进行了评估，通过多种流式细胞术方法，在精确的kinetic.Results的表达CD 64在IFN γ刺激的样品中，和CD 169在IFN α（和所有其他I型IFN）刺激的那些有统计学意义的较高。在孵育6至8小时内，通过Janus激酶/信号转导子和转导途径的激活子，由其各自的IFN直接诱导表面表达。混合这两种类型的干扰素似乎表明，他们部分抑制either.Conclusions诱导的单核细胞和中性粒细胞上的CD 164的I型和II型干扰素证实了这些标志物之间的相关性评估病毒与细菌为导向的免疫反应。
Introduction CD64 expression increases on neutrophils during bacterial infections. Recently an increase in CD169 expression has been discovered on monocytes during viral infections. Generally, interferons alpha (IFNs alpha) and IFNs gamma are key drivers of the infectious host immune response. The purpose of this study was to explore if a link exists between these IFNs and both biomarkers.Methods Whole blood samples from healthy volunteers were stimulated with either IFNs, interleukins, or infectious extracts, to mimic an infectious state. Expressions of CD64 and CD169 were assessed in these samples by multiple flow cytometry methods, over precise kinetics.Results The expression of CD64 was statistically higher in samples stimulated with IFN gamma, and CD169 in those stimulated with IFN alpha (and all other type I IFNs). Surface expressions are directly induced by their respective IFNs via Janus kinase/signal transducer and activator of transduction pathways within 6 to 8 hours of incubation. Mixing both types of IFNs seemed to indicate that they partially inhibit each other.Conclusions The induction of CD169 on monocytes and CD164 on neutrophils by type I and type II IFNs confirms the relevance of these markers for assessing between a viral- vs bacterial-oriented immune response.