Myostatin gene silenced by RNAi show a zebrafish giant phenotype
Myostatin gene silenced by RNAi show a zebrafish giant phenotype
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DOI：
10.1016/j.jbiotec.2005.04.023
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发表时间：
2005-10-10
影响因子：
4.1
通讯作者：
Estrada, MP
中科院分区：
文献类型：
--
作者：
Acosta, J;Carpio, Y;Estrada, MP
肌生长抑制素是转化生长因子-β（TGF-β）家族的成员，其作为骨骼肌发育和生长的负调节剂起作用。最近，有报道称，转基因斑马鱼表达肌肉生长抑制素前结构域表现出骨骼肌纤维数量的增加。其他新的结果表明，除了抑制增殖和分化之外，肌生成抑制素在肌肉发生过程中发挥着重要作用。我们研究了双链RNA（dsRNA）抑制斑马鱼肌生成抑制素功能的能力。通过显微注射dsRNA，获得罗非鱼肌肉生长抑制素蛋白C端268位氨基酸至末端密码子的生物活性结构域。我们在处理过的鱼中产生了增加的体重。在斑马鱼早期发育阶段注射dsRNA可引起斑马鱼的增生或肥大。此外，基因功能的干扰表现出强烈的依赖于dsRNA的量。(C)2005 Elsevier B. V.保留所有权利。
Myostatin is a member of the transforming growth factor-beta (TGF-beta) family that functions as, a negative regulator of skeletal muscle development and growth. Recently, it has been reported that the transgenic zebrafish expressing myostatin prodomain exhibited an increased number of fiber in skeletal muscle. Other novel results suggest that myostatin plays a mayor role during myogenesis, apart from inhibition of proliferation as well as differentiation, We have investigated the ability of double-stranded RNA (dsRNA) to inhibit myostatin function in the zebrafish. By microinjection dsRNA, corresponding to biologically active C-terminal domain from aminoacid 268 to end codon of tilapia myostatin protein. we produced an increased body mass in treated fish. The dsRNA injection in early development stage in zebrafish produced hyperplasia or hypertrophy. In addition, the interference of gene function showed a strong dependence on the amount of dsRNA. (C) 2005 Elsevier B.V. All rights reserved.