Heterodyne dual-polarization epi-detected CARS microscopy for chemical and topographic imaging of interfaces
Heterodyne dual-polarization epi-detected CARS microscopy for chemical and topographic imaging of interfaces
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DOI：
10.1117/12.2507636
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发表时间：
2019-03
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影响因子：
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通讯作者：
W. Langbein;Dafydd Sion Harlow;D. Regan;I. Pope;P. Borri
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作者：
W. Langbein;Dafydd Sion Harlow;D. Regan;I. Pope;P. Borri
我们介绍了一种由我们最近开发的无标签振动显微镜技术，该技术提供无背景的化学特异性图像对比度，有限的射击噪声检测和相位灵敏度，使界面的地形成像成为可能。该技术的特点是干涉外差检测外延几何的相干反斯托克斯拉曼散射（CARS），以及在同一仪器中多模态采集受激拉曼散射和正向发射的CARS强度。作为一个重要的生物学相关的应用，外差检测单个脂质双分子层的CARS成像被证明。我们表明，我们可以分辨单一的脂质双分子层，不同于双分子层，并测量其磁化率的相，这提供了纳米分辨率的双分子层的地形信息。作为一个额外的应用示例，我们展示了在玻璃-水界面处被水环境包围的硅油滴的成像，其中区分了三种不同的信号产生途径。因此，我们的外延检测外差CARS显微镜装置为激动人心的新实验铺平了道路，将振动显微镜的灵敏度和分辨率极限推向纳米级。
We present a label-free vibrational microscopy technique recently developed by us, which offers backgroundfree chemically-specific image contrast, shot-noise limited detection, and phase sensitivity enabling topographic imaging of interfaces. The technique features interferometric heterodyne detection of coherent anti-Stokes Raman scattering (CARS) in epi-geometry, as well as multi-modal acquisition of stimulated Raman scattering and forward-emitted CARS intensity in the same instrument. As an important biologically-relevant application, epi-detected heterodyne CARS imaging of individual lipid bilayers is demonstrated. We show that we can resolve a single lipid bilayer, distinct from a double bilayer, and measure the phase of its susceptibility, which provides information about the topography of the bilayer with nanometer resolution. As an additional application example, we show imaging of silicon oil droplets surrounded by an aqueous environment at the glass-water interface, where three different signal generation pathways are distinguished. Our epi-detected heterodyne CARS microscope setup thus paves the way to exciting new experiments pushing the sensitivity and resolution limits of vibrational microscopy to the nanoscale.