Effect of increasing ratio of estrogen: Androgen on proliferation of normal human prostate stromal and epithelial cells, and the malignant cell line LNCaP
Effect of increasing ratio of estrogen: Androgen on proliferation of normal human prostate stromal and epithelial cells, and the malignant cell line LNCaP
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DOI：
10.1002/pros.20327
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发表时间：
2006-01-01
期刊：
影响因子：
2.8
通讯作者：
Assinder, SJ
中科院分区：
文献类型：
--
作者：
King, KJ;Nicholson, HD;Assinder, SJ
背景在老年男性中看到的类固醇比例的变化被认为是促进前列腺疾病。本研究的目的是比较不同比例的类固醇对正常基质和上皮细胞分离株以及前列腺癌细胞系LNCaP生长的影响。改变类固醇比例对正常基质（PrSC）和上皮（PrEC）前列腺细胞以及恶性细胞系LNCaP细胞增殖的影响进行了评估。增加雌激素：双氢睾酮（DHT）和双氢睾酮：雌激素的比例，刺激PrSC增殖，增加雌激素：双氢睾酮具有最大的效果。两种类固醇均显著增加LNCaP增殖，但改变比例没有额外的影响。当细胞单独生长时，PrEC增殖不受影响，尽管存在雄激素受体（AR）和雌激素受体（ER）。当在共培养物中生长时，PrEC细胞增殖通过处理显著增加。PrSC增殖受雌激素：雄激素比例增加的刺激。由于基质细胞介导的类固醇的间接作用，刺激正常上皮细胞的增殖。相比之下，恶性前列腺癌细胞的反应有所改变。
BACKGROUND. Changes in steroid ratios seen in the aging male are thought to promote prostate disease. The aims of this study were to compare the effects of varied ratios of steroids on growth of normal stromal and epithelial cell isolates, and the prostate cancer cell line, LNCaP.METHODS. The effect of altered steroid ratios on cell proliferation of normal stromal (PrSC) and epithelial (PrEC) prostate cells, and the malignant cell line, LNCaP, were assessed.RESULTS. Increasing the ratios of both estrogen:dihydrotestosterone (DHT) and DHT:estrogen, stimulated PrSC proliferation, with increasing estrogen:DHT having the greatest effect. LNCaP proliferation was increased significantly by both steroids, but altered ratios had no additional effect. PrEC proliferation was unaffected when cells were grown alone, despite presence of androgen receptors (AR) and estrogen receptors (ER). When grown in co-culture PrEC cell proliferation was significantly increased by treatments.CONCLUSIONS. PrSC proliferation is stimulated by an increasing ratio of estrogen:androgen. Proliferation of normal epithelial cells is stimulated as a result of an indirect action of steroids mediated by stromal cells. Malignant prostate cancer cells have an altered response in comparison.