CALCIUM AS A REGULATOR OF PROLIFERATION OF NORMAL, BUT NOT OF TRANSFORMED, CHICKEN FIBROBLASTS IN A PLASMA-CONTAINING MEDIUM
CALCIUM AS A REGULATOR OF PROLIFERATION OF NORMAL, BUT NOT OF TRANSFORMED, CHICKEN FIBROBLASTS IN A PLASMA-CONTAINING MEDIUM
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DOI：
10.1073/pnas.68.2.271
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发表时间：
1971-01-01
影响因子：
11.1
通讯作者：
BALK, SD
中科院分区：
文献类型：
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作者：
BALK, SD
在含有5%热灭活鸡血浆的低钙浓度培养基中，正常的鸡成纤维细胞分裂非常缓慢，而经施密特-鲁平劳斯肉瘤病毒转化的成纤维细胞分裂速度要快得多。在同样的低钙培养基中，使用热灭活血清，而不是热灭活血浆，可以使正常细胞和转化细胞快速分裂。使用热灭活血浆而不是血清，并使用低钙培养基，可以证明正常细胞和肿瘤细胞的增殖行为之间存在显著差异，并为后者的自主性提供了基础。
In a culture medium of low calcium concentration containing 5% heat-inactivated chicken plasma, normal chicken fibroblasts divide very slowly, while their counterparts transformed with Schmidt-Ruppin Rous sarcoma virus divide much more rapidly. In the same low-calcium medium, the use of heat-inactivated serum, rather than heat-inactivated plasma, results in rapid division of both normal and transformed cells.The use of heat-inactivated plasma rather than serum, and the use of a low-calcium medium, combine to permit the demonstration of a marked difference between the proliferative behaviors of normal and neoplastic cells and suggest a basis for the autonomy of the latter.