Dissociation of biomolecules using a ultraviolet matrix-assisted laser desorption/ionisation time-of-flight/curved field reflectron tandem mass spectrometer equipped with a differential-pumped collision cell
Dissociation of biomolecules using a ultraviolet matrix-assisted laser desorption/ionisation time-of-flight/curved field reflectron tandem mass spectrometer equipped with a differential-pumped collision cell
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DOI：
10.1002/rcm.2486
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发表时间：
2006-01-01
影响因子：
2
通讯作者：
Raptakis, Emmanuel
中科院分区：
文献类型：
--
作者：
Belgacem, Omar;Bowdler, Andrew;Raptakis, Emmanuel
对配备弯曲场反射器 (CFR) 的商用基质辅助激光解吸/电离飞行时间 (MALDI-ToF) 仪器进行改造，以便对各种生物分子进行碰撞诱导解离 (CID)。将高分辨率离子门与无场区域内的碰撞池结合在一起，可以进行串联质量分析 (MS/MS)，而无需在激活前对前体离子进行减速。使用 CFR 仍然可以同时检测所有产物离子。为了测试 MS/MS 性能，在改进的仪器上分析了 ACTH（片段 1-17）、复杂的高甘露糖碳水化合物 (Man)(8)(GlcNac)(2) 和溶血磷脂酰胆碱脂质 (18:1)。与在 MALDI 四极离子阱 (QIT) 两级反射飞行时间 (ReToF) 质谱仪上获得的低能产物离子谱进行直接比较，结果表明观察到的产物离子类型存在显着差异。检测到的额外离子清楚地表明了碰撞池中发生的高能碎片过程。版权所有 (c) 2006 John Wiley & Sons, Ltd.
A commercial matrix-assisted laser desorption/ionisation time-of-flight (MALDI-ToF) instrument equipped with a curved field reflectron (CFR) was modified in order to perform collision-induced dissociation (CID) on a variety of biomolecules. The incorporation of a high-resolution ion gate together with a collision cell within the field-free region allowed tandem mass analysis (MS/MS), without the necessity to decelerate the precursor ions prior to activation. The simultaneous detection of all product ions remained possible by using the CFR. To test the MS/MS performances, ACTH (fragment 1-17), a complex high mannose carbohydrate (Man)(8)(GlcNac)(2) and a lysophosphatidylcholine lipid (18:1) were analysed on the modified instrument. Direct comparison with the low-energy product ion spectra, acquired on a MALDI quadrupole ion trap (QIT) two-stage reflectron time-of flight (ReToF) mass spectrometer, showed significant differences in the types of product ions observed. The additional ions detected were a clear indication of the high-energy fragmentation processes occurring in the collision cell. Copyright (c) 2006 John Wiley & Sons, Ltd.