Wound Matrix Stiffness Imposes on Macrophage Activation.
Wound Matrix Stiffness Imposes on Macrophage Activation.
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DOI：
10.1007/978-1-0716-0845-6_11
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Lin PH
中科院分区：
文献类型：
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作者：
Duann P;Lin PH
免疫系统依赖于两个主要途径-先天免疫和适应性免疫。巨噬细胞作为吞噬和消化入侵者的第一道免疫防线，是整合这两条途径的关键效应细胞。巨噬细胞活化在创伤修复、炎症消退和组织重塑过程中的动态可塑性是免疫功能研究中新兴的生物医学和生物工程热点，例如细胞因子和趋化因子的各种分泌以及配体及其同源受体的信号传导途径。更好地了解物理/机械和多细胞微环境对巨噬细胞功能的调节将创造创新疗法，以促进宿主防御机制并协助伤口愈合。在此，我们描述了一种简单的方法来测量功能（基因表达）的人类和小鼠巨噬细胞在机械微环境的变化，通过包埋分离的巨噬细胞在聚合透明质酸凝胶不同的伤口基质刚度。
The immune system depends on two major paths—the innate and the adaptive immunity. Macrophage, with its unique features as the first line of immune defense to engulf and digest invaders, serves as the key effector cells integrating those two paths. The dynamic plasticity of macrophage activation during wound repair, inflammation resolution, and tissue remodeling are emerging biomedical and bioengineering hot topics in immune function studies such as the various secretions of cytokines and chemokines and the signaling pathways with ligands and their cognate receptors. Better knowledge on how physical/mechanical and multicellular microenvironment on the modulation of macrophage functions will create innovative therapies to boost host defense mechanism and assist wound healing. In this, we describe an easy method to measure functions (gene expressions) of human and mouse macrophages in response to mechanical microenvironment changes by embedding isolated macrophages in polymerized hyaluronan gel with different wound matrix stiffness.