Monocytes recruited to sites of inflammation express a distinctive proinflammatory (P) phenotype.
Monocytes recruited to sites of inflammation express a distinctive proinflammatory (P) phenotype.
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募集到炎症部位的单核细胞表达独特的促炎（P）表型。
DOI：
10.1152/ajplung.1994.267.6.l786
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Campbell,EJ
中科院分区：
文献类型：
--
作者：
Owen,CA;Campbell,MA;Boukedes,SS;Campbell,EJ
只有一小部分单核细胞对趋化因子有反应。为了测试化学引诱物响应性单核细胞具有独特功能特征的可能性，我们富集或耗尽了具有促炎（P）表型的细胞的单核细胞制剂，并测试了它们对生物学相关化学引诱物的响应。我们通过三种独立技术之一制备单核细胞亚群，以最大限度地减少伪影的机会：1）通过粘附纤连蛋白消耗P单核细胞; 2）通过HLA-DR抗原阴性选择富集P单核细胞; 3）流式细胞术分选。我们通过三个参数测量了单核细胞亚群对N-甲酰-Met-Leu-Phe、C5 a、酵母聚糖激活血清和单核细胞趋化蛋白-1的反应性：1）极化，2）肌动蛋白聚合，3）定向迁移。与每个化学引诱物和每个参数，有一个显着的直接关系之间的反应性的单核细胞制剂和它们的内容P单核细胞。我们的数据表明，单核细胞从脉管系统迅速募集到炎症部位的能力是单核细胞亚群具有独特的嗜中性粒细胞样促炎表型的特性。
Only a minor proportion of monocytes responds to chemoattractants. To test the possibility that chemoattractant-responsive monocytes have distinctive functional characteristics, we enriched or depleted monocyte preparations for cells having a proinflammatory (P) phenotype and tested their responses to biologically relevant chemoattractants. We prepared monocyte subpopulations by one of three independent techniques to minimize the chances of artifacts: 1) depletion of P monocytes by adherence to fibronectin; 2) enrichment for P monocytes by negative selection for HLA-DR antigen; and 3) flow cytometric sorting. We measured responsiveness of monocyte subpopulations to N-formyl-Met-Leu-Phe, C5a, zymosan-activated serum, and monocyte chemoattractant protein-1 by three parameters: 1) polarization, 2) actin polymerization, and 3) directed migration. With each chemoattractant and each parameter, there was a striking direct relationship between the responsiveness of the monocyte preparations and their content of P monocytes. Our data indicate that the capacity of monocytes to be recruited rapidly from the vasculature into sites of inflammation is a property of a subpopulation of monocytes with a distinctive, neutrophil-like proinflammatory phenotype.