Protective effect of hypotaurine against oxidative stress-induced cytotoxicity in rat placental trophoblasts
Protective effect of hypotaurine against oxidative stress-induced cytotoxicity in rat placental trophoblasts
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DOI：
10.1016/j.placenta.2015.02.014
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发表时间：
2015-06-01
期刊：
影响因子：
3.8
通讯作者：
Nakashima, E.
中科院分区：
文献类型：
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作者：
Nishimura, T.;Duereh, M.;Nakashima, E.
前言：次牛磺酸是牛磺酸的前体，也是一种抗氧化剂，与母体血浆相比，它主要集中在胎儿血浆中。Ezrin(Vil2)基因敲除小鼠胎儿血浆中的次牛磺酸显著降低，胎儿出现宫内发育迟缓。本研究旨在探讨胎盘滋养层细胞维持细胞下牛磺酸水平的机制，以及亚牛磺酸对过氧化氢(H_2O_2)诱导的氧化应激的影响。方法：分析胎盘滋养层细胞外液中的亚牛磺酸转运及亚牛磺酸对胎盘滋养层细胞的抗氧化作用。结果：胎盘滋养层细胞中的亚牛磺酸在胎盘滋养层细胞中集中分布，在添加胎牛血清(FBS)而不是正常胎牛血清的条件下，细胞内的亚牛磺酸水平显著降低。当向培养基中加入亚牛磺酸时，亚牛磺酸水平几乎完全恢复，这表明细胞内亚牛磺酸主要是通过细胞外液跨质膜运输而不是通过生物合成来提供的。次牛磺酸对过氧化氢诱导的TR-TBT 18d-1细胞氧化损伤具有保护作用。次牛磺酸处理的tr-TBT 18d-1细胞提高了对羟基自由基和过氧化自由基的抗氧化能力。经亚牛磺酸处理后，细胞内过氧化氢诱导的细胞内羟基自由基浓度显著降低。结论：亚牛磺酸主要通过胞外液跨质膜转运的方式提供给胎盘滋养层细胞，并可能通过清除活性氧而发挥细胞保护作用。(C)2015爱思唯尔有限公司。保留所有权利。
Introduction: Hypotaurine is a precursor of taurine and an antioxidant, and is concentrated in fetal plasma compared to maternal plasma. Hypotaurine is significantly decreased in fetal plasma of ezrin (Vil2) knock-out mice, and fetuses show intrauterine growth retardation. The aim of this study was to characterize the mechanism through which cellular hypotaurine level is maintained in placental trophoblasts, and the effect of hypotaurine on oxidative stress induced by hydrogen peroxide (H2O2).Methods: Hypotaurine transfer from extracellular fluid and antioxidant effect of hypotaurine were analyzed in rat placental trophoblast TR-TBT 18d-1 cells.Results: We found that hypotaurine is concentrated into rat placental trophoblast TR-TBT 18d-1 cells, and the level of hypotaurine was markedly reduced by culture in medium supplemented with dialyzed fetal bovine serum (FBS) instead of normal FBS. The hypotaurine level recovered almost completely when hypotaurine was added to the culture medium, indicating that intracellular hypotaurine is predominantly supplied by transport across the plasma membrane from extracellular fluid rather than by biosynthesis. Hypotaurine showed a cytoprotective effect against H2O2-induced oxidative damage in TR-TBT 18d-1 cells. Hypotaurine treatment of TR-TBT 18d-1 cells increased antioxidant capacity against hydroxyl radical and peroxyl radical. The concentration of intracellular hydroxyl radical induced by H2O2 in TR-TBT 18d-1 cells was significantly reduced by hypotaurine treatment.Discussion: These results indicate that intracellular hypotaurine is mainly supplied to placental trophoblasts by transfer from extracellular fluid across the plasma membrane, and may play a role in cell protection by scavenging reactive oxygen species. (C) 2015 Elsevier Ltd. All rights reserved.