Desulfotomaculum arcticum sp nov., a novel spore-formin, moderately thermophilic, sulfate-reducing bacterium isolated from a permanently cold fjord sediment of Svalbard
Desulfotomaculum arcticum sp nov., a novel spore-formin, moderately thermophilic, sulfate-reducing bacterium isolated from a permanently cold fjord sediment of Svalbard
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DOI：
10.1099/ijs.0.64058-0
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发表时间：
2006-04-01
影响因子：
2.8
通讯作者：
Jorgensen, BB
中科院分区：
文献类型：
--
作者：
Vandieken, V;Knoblauch, C;Jorgensen, BB
菌株15 T是从斯瓦尔巴特群岛永久寒冷的峡湾沉积物中分离出的一种新的孢子形成、硫酸盐还原细菌。硫酸盐可被亚硫酸盐或硫代硫酸盐代替。氢、甲酸盐、乳酸盐、丙酸盐、丁酸盐、己酸盐、甲醇、乙醇、丙醇、丁醇、丙酮酸盐、苹果酸盐、琥珀酸盐、富马酸盐、脯氨酸、丙氨酸和甘氨酸在硫酸盐存在下用作电子供体。以丙酮酸为唯一底物进行生长。在pH 7（.）1-7（.）5和1-1（.）5%NaCl和0（.）4%MgCl_2，菌株15 T生长在26 ~ 46（.）最佳生长温度为44 ℃。因此，菌株15 T显然不能在其分离的北极沉积物的原位温度下生长，并提出其以孢子形式存在于沉积物中。DNA G+C含量为48（.）9mol%。菌株15 T与嗜热脱硫肠菌MLFT的亲缘关系最近（16 S rRNA基因序列相似性为93-5%）。菌株15 T代表一个新种，拟命名为北极脱硫肠状菌Desulfotomaculum arcticum sp. nov..模式菌株是菌株15 T（=DSM 17038（T）=jCM 12923（T））。
Strain 15 T is a novel spore-forming, sulfate-reducing bacterium isolated from a permanently cold fjord sediment of Svalbard. Sulfate could be replaced by sulfite or thiosulfate. Hydrogen, formate, lactate, propionate, butyrate, hexanoate, methanol, ethanol, propanol, butanol, pyruvate, malate, succinate, fumarate, proline, alanine and glycine were used as electron donors in the presence of sulfate. Growth occurred with pyruvate as sole substrate. Optimal growth was observed at pH 7(.)1-7(.)5 and concentrations of 1-1(.)5% NaCl and 0(.)4% MgCl2, Strain 15 T grew between 26 and 46(.)5 degrees C and optimal growth occurred at 44 degrees C. Therefore, strain 15 T apparently cannot grow at in situ temperatures of Arctic sediments from where it was isolated, and it was proposed that it was present in the sediment in the form of spores. The DNA G+C content was 48(.)9 mol%. Strain 15 T was most closely related to Desulfotomaculum thermosapovorans MLFT (93-5% 16S rRNA gene sequence similarity). Strain 15 T represents a novel species, for which the name Desulfotomaculurn arcticum sp. nov. is proposed. The type strain is strain 15 T (=DSM 17038(T)=jCM 12923(T)).