PRESENCE OF PHOSPHORYLATED O-RIBOSYL-ADENOSINE IN T-PSI-STEM OF YEAST METHIONINE INITIATOR TRANSFER-RNA
PRESENCE OF PHOSPHORYLATED O-RIBOSYL-ADENOSINE IN T-PSI-STEM OF YEAST METHIONINE INITIATOR TRANSFER-RNA
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DOI：
10.1093/nar/17.3.865
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发表时间：
1989-02-11
影响因子：
14.9
通讯作者：
GEHRKE, CW
中科院分区：
文献类型：
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作者：
DESGRES, J;KEITH, G;GEHRKE, CW
我们在这篇论文中报告了两个未知的核苷G* 和[A*]的分离和表征位于T-。酵母甲硫氨酸起始物tRNA的茎，使用HPLC方案、真实的时间UV吸收光谱和通过电子轰击或快速原子轰击的运行后质谱的组合手段。65位的G* 核苷被鉴定为未修饰的鸟苷。通过将其色谱、UV-光谱和质谱性质与真正的O-α-腺苷的色谱、UV-光谱和质谱性质进行比较，将位置64上的未知[A*]的结构表征为O-核糖基-腺苷的异构形式。呋喃核糖基（1”→从生物合成的聚（腺苷二磷酸核糖）中分离的2“）-腺苷。我们的研究还提供了位于这种新的修饰核苷[A*]上的磷酸单酯基团存在的证据，当通过离子交换色谱法从酵母引发剂tRNAMet的酶促水解中分离时，没有磷酸酶处理。
We report in this paper on isolation and characterization of two unknown nucleosides G* and [A*] located in the T-.PSI.-stem of yeast methionine initiator tRNA, using the combined means of HPLC protocols, real time UV-absorption spectrum, and post-run mass spectrometry by electron impact or fast atom bombardment. The G* nucleoside in position 65 was identified as unmodified guanosine. The structure of the unknown [A*] in position 64 was characterized as an isomeric form of O-ribosyl-adenosine by comparison of its chromatographic, UV-spectral and mass spectrometric properties with those of authentic O-.alpha.-ribofuranosyl(1" .fwdarw. 2'')-adenosine isolated from biosynthetic poly(adenosine diphosphate ribose). Our studies also brought evidence for the presence of a phosphorylmonoester group located on this new modified nucleoside [A*], when isolated by ion exchange chromatography from enzymic hydrolysis of yeast initiator tRNAMet without phosphatase treatment.