The endophytic bacterial entomopathogen Serratia marcescens promotes plant growth and improves resistance against Nilaparvata lugens in rice.
The endophytic bacterial entomopathogen Serratia marcescens promotes plant growth and improves resistance against Nilaparvata lugens in rice.
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DOI：
10.1016/j.micres.2021.126956
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发表时间：
2021-12
影响因子：
6.7
通讯作者：
H. Niu;Yang Sun;Zhichun Zhang;Dongxiao Zhao;Na Wang;Lihua Wang;Huifang Guo
中科院分区：
文献类型：
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作者：
H. Niu;Yang Sun;Zhichun Zhang;Dongxiao Zhao;Na Wang;Lihua Wang;Huifang Guo
昆虫病原细菌通常用作生物制剂来控制不同的昆虫害虫。然而，细菌性昆虫病原菌作为植物内生菌在调节植物生长和抗虫性方面的作用知之甚少。本研究以粘质沙雷氏菌（Serratia marcescensS-JS 1）为病原菌，通过接种水稻种子，研究了粘质沙雷氏菌S-JS 1对水稻生长及对褐飞虱抗性的影响。此外，诱导防御相关的次生代谢产物的细菌进行了评估，通过GC-MS/MS。我们表明，S-JS 1能够内生定殖种子接种后的水稻幼苗的根和芽。与未接种的植物相比，定殖的植物显示出增加的种子发芽（9.4 - 13.3%）、根（8.2 - 36.4%）和芽长度（4.1 - 22.3%）以及根（26.7 - 69.3%）和芽鲜重（19.0 - 49.0%）。我们还鉴定了S-JS 1产生的吲哚-3-乙酸，这可能与促进水稻植物生长有关。在一个二选一的测试中，N。褐飞虱成虫更喜欢取食未处理的对照植物而不是用S-JS 1处理的植物。在无选择摄食试验中，N.取食S-JS 1处理植株的绿僵菌的生长速率显著低于取食未处理植株的绿僵菌。此外，用109cfu/mL S-JS 1处理的种子导致次生代谢产物水平升高，这可能与N.水稻对褐飞虱的抗性。因此，我们认为昆虫病原细菌S. marcescensbe被认为是一种潜在的有前途的内生菌，可用于昆虫害虫综合管理的创新策略。
Entomopathogenic bacteria are commonly used as biological agents to control different insect pests. However, little is known about the role of bacterial entomopathogens as endophytes in regulating both plant growth and resistance against insect pests. Here, we applied the entomopathogenic bacteriumSerratia marcescensS-JS1 via rice seed inoculation and evaluated its effects on host plant growth and resistance against the rice pestNilaparvata lugens. Furthermore, the induction of defense-related secondary metabolites by the bacterium was assessed by GC–MS/MS. We showed that S-JS1 was able to endophytically colonize the roots and shoots of rice seedlings following seed inoculation. Colonized plants showed increased seed germination (9.4–13.3 %), root (8.2–36.4 %) and shoot lengths (4.1–22.3 %), and root (26.7–69.3 %) and shoot fresh weights (19.0–49.0 %) compared to plants without inoculation. We also identified the production of indole-3-acetic acid by S-JS1, which is likely involved in enhancing rice plant growth. In a two-choice test,N. lugensadults preferred to feed on untreated control plants than on plants treated with S-JS1. In the no-choice feeding tests, the survival ofN. lugensnymphs that fed on S-JS1-treated plants was significantly lower than that of nymphs that fed on untreated plants. Additionally, seeds treated with 109cfu/mL S-JS1 resulted in elevated levels of secondary metabolites, which may be associated withN. lugensresistance in rice plants. Therefore, we suggest that the entomopathogenic bacteriumS. marcescensbe considered a potentially promising endophyte for use in an innovative strategy for the integrated management of insect pests.