Influence of Coating and Size of Magnetic Nanoparticles on Cellular Uptake for In Vitro MRI.
Influence of Coating and Size of Magnetic Nanoparticles on Cellular Uptake for In Vitro MRI.
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DOI：
10.3390/nano11112888
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发表时间：
2021-10-28
期刊：
影响因子：
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通讯作者：
Ayuso-Sacido Á
中科院分区：
文献类型：
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作者：
Cortés-Llanos B;Ocampo SM;de la Cueva L;Calvo GF;Belmonte-Beitia J;Pérez L;Salas G;Ayuso-Sacido Á
氧化铁纳米粒子（IONP）是磁共振成像（MRI）中对比度增强的合适材料。它们的潜在临床应用范围从诊断到治疗和后续治疗。然而，为了将该技术的应用扩展到不同类型的癌症治疗，需要更深入地了解 IONP、培养基和细胞之间的相互作用。为了获得对这些相互作用的新见解，我们用相同的无机核和五种不同的涂层制备了一组 IONP，以研究它们在不同生理介质中的聚集和相互作用，以及它们的细胞标记效率。然后，使用第二组 IONP（具有六种不同的核心尺寸和相同的涂层）来研究核心尺寸如何影响体外细胞标记和 MRI。在这里，悬浮在生物介质中的 IONP 的涂层和分子粘附被部分去除。 FBS 浓度改变所有类型 IONP 的标记，并且流体动力学尺寸 ≥ 300 nm 使用离心介导的内化 (CMI) 提供最大的标记。 MRI 结果的最佳对比度需要涂有二巯基丁二酸 (DMSA) 的核心尺寸范围为 12–14 nm，产生的 R2* 值分别为 393.7 s−1 和 428.3 s−1。这些发现将有助于将 IONP 作为阴性造影剂引入临床。
Iron oxide nanoparticles (IONPs) are suitable materials for contrast enhancement in magnetic resonance imaging (MRI). Their potential clinical applications range from diagnosis to therapy and follow-up treatments. However, a deeper understanding of the interaction between IONPs, culture media and cells is necessary for expanding the application of this technology to different types of cancer therapies. To achieve new insights of these interactions, a set of IONPs were prepared with the same inorganic core and five distinct coatings, to study their aggregation and interactions in different physiological media, as well as their cell labelling efficiency. Then, a second set of IONPs, with six different core sizes and the same coating, were used to study how the core size affects cell labelling and MRI in vitro. Here, IONPs suspended in biological media experience a partial removal of the coating and adhesion of molecules. The FBS concentration alters the labelling of all types of IONPs and hydrodynamic sizes ≥ 300 nm provide the greatest labelling using the centrifugation-mediated internalization (CMI). The best contrast for MRI results requires a core size range between 12–14 nm coated with dimercaptosuccinic acid (DMSA) producing R2* values of 393.7 s−1 and 428.3 s−1, respectively. These findings will help to bring IONPs as negative contrast agents into clinical settings.