Articular chondrocytes lose their proliferative activity with aging yet can be restimulated by PTH‐(1–84), PGE1, and Dexamethasone
Articular chondrocytes lose their proliferative activity with aging yet can be restimulated by PTH‐(1–84), PGE1, and Dexamethasone
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关节软骨细胞随着衰老而失去增殖活性，但可以通过 PTH-(1-84)、PGE1 和地塞米松重新刺激
DOI：
10.1002/jbmr.5650040413
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发表时间：
1989
影响因子：
6.2
通讯作者：
M. Silbermann
中科院分区：
文献类型：
--
作者：
E. Livne;A. Weiss;M. Silbermann
小鼠下颌骨髁突在6个月龄时发生自发性退行性变化，因此为与年龄相关性骨关节炎发作和进展相关过程的研究提供了良好的体内模型。此外，该关节提供了一个适当的系统来研究关节软骨对老年激素和局部生长因子反应的潜力。本研究检查了（1）小鼠下颌髁突关节软骨细胞[3 H]胸苷掺入的年龄相关变化，以及（2）全身和局部因素对组织恢复DNA合成能力的影响。在PTH-（1-84）（2 μ g/ml）、PGE 1（20 μ g/ml）、地塞米松（10−7 M）和MSA（5 μg/ml）存在下培养3 - 18月龄雌性CW-1小鼠的髁，并用[3 H]胸苷同时标记。对放射自显影进行定量分析，结果显示：（1）关节软骨标记指数出现显著（p < 0.01）年龄相关性降低（-80%）;（2）在体外用PTH-（1-84）、PGE 1和地塞米松处理后，旧组织恢复DNA合成的能力。伴随的定量掺入研究进一步证实了放射自显影结果。因此，这些因子对参与自发性骨关节炎晚期的衰老软骨细胞具有直接刺激作用。
Mouse mandibular condyles develop spontaneous degenerative changes by 6 months of age, hence providing a good in vivo model for studies related to processes associated with the onset and progression of age‐related osteoarthritis. Further, this joint provides an appropriate system to investigate the potential of articular cartilage to respond to hormones and local growth factors in old age. The present study examined (1) the age‐related changes in [3H]thymidine incorporation by articular chondrocytes in the mouse mandibular condyle, and (2) the effect of systemic and local factors upon the tissue's ability to resume DNA synthesis. Condyles of female CW‐1 mice ranging from 3 to 18 months of age were cultured in the presence of PTH‐(1–84) (2 μ/g/ml), PGE1 (20 μ/ml), dexamethasone (10−7 M), and MSA (5 μg/ml) and were concomitantly labeled with [3H]thymidine. Autoradiographs were analyzed quantitatively and revealed (1) a significant (p < 0.01) age‐related decrease (–80%) in the labeling index of the articular cartilage, and (2) the ability of old tissues to resume DNA synthesis following in vitro treatment with PTH‐(1–84), PGE1, and dexamethasone. Concomitant quantitative incorporation studies further substantiated the autoradiographic findings. Hence, these factors possess a direct stimulatory effect upon senescent chondrocytes involved in an advanced stage of spontaneous osteoarthritis.
合成人甲状旁腺激素对鸡骨骺生长板软骨细胞原代培养物碱性磷酸酶活性水平和富含碱性磷酸酶基质囊泡形成的影响。
DOI：
--
发表时间：
1986
期刊：
Bone and mineral
影响因子：
--
作者：
Chin,JE;Schalk,EM;Kemick,ML;Wuthier,RE
通讯作者：
Wuthier,RE