Transport of sodium, potassium, and calcium across rabbit polymorphonuclear leukocyte membranes. Effect of chemotactic factor.
Transport of sodium, potassium, and calcium across rabbit polymorphonuclear leukocyte membranes. Effect of chemotactic factor.
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钠，钾和钙的转运跨兔多形核白细胞膜。趋化因子的影响。
DOI：
10.1083/jcb.73.2.428
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发表时间：
1977-05
期刊：
影响因子：
--
通讯作者：
Sha'afi RI
中科院分区：
文献类型：
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作者：
Naccache PH;Showell HJ;Becker EL;Sha'afi RI
兔腹膜多形核白细胞 (PMN) 质膜对 Na+、K+ 和 Ca2+ 的转运特性已得到表征。硅油离心技术的使用提供了一种快速可靠的方法来测量这些细胞中的离子通量。研究发现 Na+ 和 K+ 穿过 PMN 膜的移动速度很快。 Na+ 的单火稳态通量值（以毫克当量/升细胞 X 分钟为单位）约为 3.0，K+ 约为 7.4。 Ouabian 抑制 K+ 流入和 Na+ 流出，后者也依赖于细胞外钾的存在。发现 45Ca 流入的速率常数（以 min-1 为单位）为 0.05，45Ca 流出的速率常数为 0.04。研究发现合成趋化因子甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸 (FMLP) 在浓度低至 10(-10)M 时会影响 Na+、K+ 和 Ca2+ 的通量。 FMLP 诱导 PMN 质膜对 22Na 的渗透性大幅且快速增加。还注意到 42K 流入和 22Na 流出的较小且延迟的增强。一些证据表明，后者的发现是 22Na 流入量增加的结果。 FMLP 还刺激 45Ca 流入和流出。在存在 0.25 mM 细胞外钙的情况下，FMLP 诱导细胞相关 45Ca 的稳态水平增加。然而，在存在 0.01 mM 细胞外钙的情况下，FMLP 会诱导细胞相关 45Ca 的稳态水平短暂降低。 FMLP 浓度与其对阳离子通量影响的关系曲线与其增强迁移的曲线非常相似。
The transport properties of the rabbit peritoneal polymorphonuclear leukocyte (PMN) plasma membrane to Na+, K+, and Ca2+ have been characterized. The use of a silicone oil centrifugation technique provided a rapid and reliable method for measuring ion fluxes in these cells. Na+ and K+ movements across PMN membranes were found to be rapid. The value for the unifirectional steady-state fluxes (in meq/liter cell X min) were of the order of 3.0 for Na+ and 7.4 for K+. Ouabian inhibited both K+ influx and Na+ efflux, the latter being also dependent on the presence of extracellular potassium. The rate constant (in min-1) for 45Ca influx was found to be .05 and that for 45Ca efflux .04. The synthetic chemotactic factor formyl-methionyl-leucyl- phenylalanine (FMLP) was found to affect the fluxes of Na+, K+, and Ca2+ at concentrations as low as 10(-10)M. FMLP induced a large and rapid increase in the permeability of the PMN plasma membrane to 22Na. Smaller and delayed enhancements of 42K influx and 22Na efflux were also noted. Some evidence that the latter findings are a consequence of the increased 22Na influx is presented. 45Ca influx and efflux were also stimulated by FMLP. In the presence of 0.25 mM extracellular calcium, FMLP induced an increase in the steady-state level of cell- associated 45Ca. In the presence of .01 mM extracellular calcium, however, a transient decrease in the steady-state level of cell- associated 45Ca was induced by FMLP. The curves relating the concentration of FMLP to its effects on cation fluxes are very similar to those found for its enhancement of migration.