Imaging Cells in Flow Cytometer Using Spatial-Temporal Transformation.
Imaging Cells in Flow Cytometer Using Spatial-Temporal Transformation.
复制标题
DOI：
10.1038/srep13267
复制
发表时间：
2015-08-18
影响因子：
4.6
通讯作者：
Lo YH
中科院分区：
文献类型：
--
作者：
Han Y;Lo YH
流式细胞仪测量荧光和光散射，并且当细胞在流体流中流动通过激发光束时分析大量单细胞的多个物理特性。尽管流式细胞仪由于其单细胞分辨率和高通量而具有巨大的统计能力，但它们不产生关于显微镜提供的细胞形态或空间分辨率的信息，这是几乎所有流式细胞仪中缺少的非常想要的特征。在本文中，我们发明了一种时空转换的方法，提供流式细胞仪与细胞成像能力。该方法使用数学算法和空间滤波器作为给予流式细胞仪成像能力所需的唯一硬件。代替CCD或在任何成像系统中发现的任何百万像素相机，我们使用PMT检测器在流式细胞仪中获得快速移动细胞的高质量图像，从而以与现有细胞仪完全兼容的方式获得高通量。为了证明这一概念，我们展示了在微流体通道中以0.2 m/s的速度行进的细胞的细胞成像，对应于每秒约1，000个细胞的吞吐量。
Flow cytometers measure fluorescence and light scattering and analyze multiple physical characteristics of a large population of single cells as cells flow in a fluid stream through an excitation light beam. Although flow cytometers have massive statistical power due to their single cell resolution and high throughput, they produce no information about cell morphology or spatial resolution offered by microscopy, which is a much wanted feature missing in almost all flow cytometers. In this paper, we invent a method of spatial-temporal transformation to provide flow cytometers with cell imaging capabilities. The method uses mathematical algorithms and a spatial filter as the only hardware needed to give flow cytometers imaging capabilities. Instead of CCDs or any megapixel cameras found in any imaging systems, we obtain high quality image of fast moving cells in a flow cytometer using PMT detectors, thus obtaining high throughput in manners fully compatible with existing cytometers. To prove the concept, we demonstrate cell imaging for cells travelling at a velocity of 0.2 m/s in a microfluidic channel, corresponding to a throughput of approximately 1,000 cells per second.