The production of 2n pollen in rose
The production of 2n pollen in rose
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DOI：
10.1007/s10681-006-9136-1
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发表时间：
2006-09-01
期刊：
影响因子：
1.9
通讯作者：
Gudin, S.
中科院分区：
文献类型：
--
作者：
Crespel, L.;Ricci, S. C.;Gudin, S.
根据二倍体和四倍体月季品种产生的单倍体和二倍体花粉的大小差异，从53个二倍体杂种群体中鉴定出2n花粉产生者，所述二倍体杂种群体来自四倍体现代栽培品种的单倍体化衍生的双单倍体月季和二倍体物种R.巫术崇拜。在2002年，2003年和2004年估计的2n花粉生产者的频率。高度可变的频率被发现i）在人群中; ii）在观察年（年与年之间和同一年的不同月份之间）。2n花粉产量的变化可能与环境波动有关。随机选取10个杂种进行了减数分裂的细胞学分析。在减数分裂异常，导致2n花粉形成，三合会（包含一个2n小孢子在一个极点和两个n小孢子在其他）所造成的异常纺锤体的几何形状经常被观察到。2n花粉形成的模式在遗传上相当于第一次分裂休眠（FDR）机制。FDR 2n花粉从二倍体亲本2n花粉生产者向四倍体后代传递高比例的杂合性。
Based on the size differences found between haploid and diploid pollen produced by diploid and tetraploid rose cultivars, respectively, 2n pollen producers were identified in a population of 53 diploid hybrids from a cross between a dihaploid rose, derived from the haploidization of a tetraploid modern cultivar and the diploid species R. wichuraiana. Frequency of 2n pollen producers was estimated in 2002, 2003 and 2004. Highly variable frequencies were found i) within population; ii) during years of observation (between years and between different months in the same year). The variation of 2n pollen production could be related to environmental fluctuations. A cytological analysis of male meiosis was carried out in 10 hybrids randomly chosen. Among meiotic abnormalities leading to 2n pollen formation, triads (containing a 2n microspore at one pole and two n microspores at the other) resulting from abnormal spindle geometry were frequently observed. The mode of 2n pollen formation is genetically equivalent to a First Division Restitution (FDR) mechanism. FDR 2n pollen transmits a high percentage of the heterozygosity from the diploid parent - 2n pollen producer-to the tetraploid offspring.