Electrochemical Genotyping by Using Two Ferrocene/Isoquinoline-Connected DNA Probes with Different Redox Potentials on a Single Electrode
Electrochemical Genotyping by Using Two Ferrocene/Isoquinoline-Connected DNA Probes with Different Redox Potentials on a Single Electrode
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DOI：
10.1002/chem.200900368
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Inouye, Masahiko
中科院分区：
文献类型：
--
作者：
Ikeda, Reona;Kitagawa, Satoshi;Inouye, Masahiko
研究了在单电极上用两个不同氧化还原电位的二茂铁/异喹啉连接的DNA探针进行电化学基因分型的方法。将100μm Iq*-ADH 7磷酸盐缓冲液溶液置于市售金电极上，并对其进行清洁。将电极在室温下在高湿度下保持在密闭容器中90分钟。用缓冲溶液冲洗后，将用DNA探针修饰的金电极在室温下浸泡在缓冲溶液的1 mM 6-巯基己烷1- 0 l溶液中90分钟，然后依次用Milli-Q水和缓冲溶液彻底洗涤。发现与匹配的Iq * 1-1相比，错配的双链体Iq* 1-2显示出增强的弹性弯曲，如同来自Iq 1的双链体一样。该电化学基因分型方法可通过使用两种具有不同氧化还原电位的DNA探针准确、快速地进行。
An electrochemical genotyping by using two ferrocene/isoquinoline-connected DNA probes with different redox potentials on a single electrode was investigated. A solution of 100μm Iq*-ADH7 phosphate buffer was placed on a commercially available gold electrode, which was cleaned. The electrode was kept in a closed container under high humidity for 90 mm at room temperature. After rinsed with a buffer solution, the gold electrode modified with DNA probes was soaked in a 1 mM 6-mercaptohexan 1-0l solution of a buffer solution for 90 mm at room temperature, and then thoroughly washed with Milli-Q water and the buffer solution successively. The mismatched duplex Iq* 1-2 was found to reveal an enhanced bending elastically compared with the matched Iq* 1-1 as the duplexes from Iq1 did. This electrochemical genotyping could be conducted accurately and rapidly by using the two DNA probes, which possess different redox potentials.