Matrix Segregation as the Major Cause for Sample Inhomogeneity in MALDI Dried Droplet Spots
Matrix Segregation as the Major Cause for Sample Inhomogeneity in MALDI Dried Droplet Spots
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DOI：
10.1007/s13361-014-0913-0
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发表时间：
2014-08-01
影响因子：
3.2
通讯作者：
Weidner, Steffen M.
中科院分区：
文献类型：
--
作者：
Gabriel, Stefan J.;Schwarzinger, Clemens;Weidner, Steffen M.
使用光学显微镜、MALDI成像质谱法（MALDI MSI）和IR成像光谱法分析干燥液滴MALDI样品点中的偏析与基质-样品比有关。在这种情况下，不同的聚合物/基质/溶剂系统通常应用于合成聚合物的分析进行了研究。使用典型的基质浓度（10 mg/mL（-1））几乎在每种情况下都会产生环形图案，而更高浓度的基质溶液总是导致均匀的样品斑点层。数据显示，偏析主要是由基质运输在干燥液滴，而聚合物偏析似乎只是次要的。
The segregation in dried droplet MALDI sample spots was analyzed with regard to the matrix-to-sample ratio using optical microscopy, MALDI imaging mass spectrometry (MALDI MSI) and IR imaging spectroscopy. In this context, different polymer/matrix/solvent systems usually applied in the analysis of synthetic polymers were investigated. The use of typical matrix concentrations (10 mg mL(-1)) in almost every case resulted in ring patterns, whereas higher concentrated matrix solutions always led to homogeneous sample spot layers. The data revealed that segregation is predominantly caused by matrix transport in the drying droplet, whereas polymer segregation seems to be only secondary.