Mechanism and enhancement of the surface stress caused by a small-molecule antigen and antibody binding.
Mechanism and enhancement of the surface stress caused by a small-molecule antigen and antibody binding.
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DOI：
10.1016/j.bios.2013.03.086
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发表时间：
2013-10
影响因子：
12.6
通讯作者：
Shangquan Wu;Tiegui Nan;Changguo Xue;T. Cheng;Hong Liu;Baomin Wang;Qingchuan Zhang;Xiaoping Wu
中科院分区：
文献类型：
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作者：
Shangquan Wu;Tiegui Nan;Changguo Xue;T. Cheng;Hong Liu;Baomin Wang;Qingchuan Zhang;Xiaoping Wu
由生物化学相互作用产生的微悬臂弯曲可以有广泛的应用，从高通量的分子检测到生物致动。然而，引起弯曲的生物化学诱导的表面应力的来源是一个很大的科学争论和兴趣的主题。与生物大分子抗原和抗体结合引起的表面压缩应力不同，这里我们证明了小分子抗原和抗体结合在表面上会产生张应力。我们认为张应力是由抗体构象变化引起的，这种变化表现为Fab臂运动，暴露了由于抗原结合而导致的抗体的C1q结合部位。研制了一种检测氯嘧磺隆(CE)的微悬臂梁免疫传感器。我们发现，定向固定化的抗体比随机固定化的抗体产生更大的合成表面应力。表面与抗体之间的连接体长度对应力传递起着重要作用。长度越短，表面应力越大。这些机制和原则将为设计显著降低小分子检测下限的装置和涂层奠定基础，并可能对我们对抗原和抗体结合的理解产生影响。
Generation of microcantilever bending from biochemical interactions can have wide applications, ranging from high-throughput molecular detection to bioactuation. However, the origin of the biochemically induced surface stress causing the bending is a subject of much scientific debate and interest. Unlike a compressive surface stress caused by biomacromolecule antigen and antibody binding, here we show that a small molecule antigen and antibody binding on the surface gives rise to a tensile stress. We propose that the tensile stress is induced by antibody conformational change which manifests itself as Fab arm motion that exposes the C1q binding site of the antibody due to antigen binding. A microcantilever immunosensor was developed for the detection of Chlorimuron-ethyl (CE). We found that antibodies with oriented immobilization induce a greater resultant surface stress than those with random immobilization. The length of linker between the surface and the antibody plays an important role on the stress transmission. The shorter the length, the greater the surface stress. These mechanism and principles will underpin the design of devices and coatings to significantly lower the small molecule detection limit and may also have an impact on our understanding of antigen and antibody binding.