In vivo degradation rate of alginate-chitosan hydrogels influences tissue repair following physeal injury
In vivo degradation rate of alginate-chitosan hydrogels influences tissue repair following physeal injury
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DOI：
10.1002/jbm.b.34580
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发表时间：
2020-02-08
影响因子：
3.4
通讯作者：
Payne, Karin A.
中科院分区：
文献类型：
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作者：
Erickson, Christopher B.;Newsom, Jake P.;Payne, Karin A.
生长板是儿童长骨中的一种软骨组织，负责骨骼的延长。Physeal损伤可以用被称为“骨条”的骨修复组织来愈合，这可能会导致生长畸形。目前的治疗方法包括手术切除骨条和插入惰性材料，以期防止骨条重新形成和保留骨延长。然而，这些材料经常失败，骨条通常会恢复。本研究探讨了海藻酸盐-壳聚糖水凝胶作为介入性材料在大鼠骨赘损伤模型中阻断骨条形成的作用。进一步研究了生物材料的性能，如基质硬度、渗透性和降解率。不同比例的海藻酸盐：壳聚糖凝胶在有或没有钙交联的情况下，测试了它们对骨棒形成的抑制和对损伤生长板的修复。海藻酸盐：壳聚糖混合(A)90：10与钙(90：10+Ca)；(B)50：50与钙(50：50+Ca)；(C)50：50不含钙(50：50-Ca)；(D)50：50用辐照海藻酸盐(IA)和不含钙。我们发现修复组织主要由海藻酸盐-壳聚糖水凝胶的体内降解率决定。90：10+Ca具有较慢的降解速度，防止了细胞的渗透，在具有更柔软、更具渗透性的材料特性的同时，产生了最多的骨棒组织。IA具有最快的降解速度，表现出高度的细胞渗透，并产生最多的软骨样组织，同时具有更坚硬、更低渗透性的材料特性。我们的结果表明，体内生物材料的降解率是一个动态属性，可以优化以影响植骨损伤的细胞命运和组织修复。
The physis is a cartilaginous tissue in children's long bones that is responsible for bone elongation. Physeal injuries can heal with bony repair tissue known as a "bony bar," and this can cause growth deformities. Current treatments involve surgical resection of the bony bar and insertion of inert materials in hopes of preventing bony bar re-formation and preserving bone elongation. However, these materials frequently fail and the bony bar commonly returns. This study investigated alginate-chitosan hydrogels as interpositional materials to block bony bar formation in a rat model of physeal injury. Further, biomaterial properties such as substrate stiffness, permeability, and degradation rate were studied. Different ratio alginate:chitosan hydrogels with or without calcium cross-linking were tested for their inhibition of bony bar formation and restoration of the injured physis. Alginate:chitosan were mixed (a) 90:10 with calcium (90:10 + Ca); (b) 50:50 with calcium (50:50 + Ca); (c) 50:50 without calcium (50:50 - Ca); and (d) 50:50 made with irradiated alginate (IA) and without calcium. We found that repair tissue was determined primarily by the in vivo degradation rate of alginate-chitosan hydrogels. 90:10 + Ca had a slow degradation rate, prevented cellular infiltration, and produced the most bony bar tissue while having softer, more permeable material properties. IA had the fastest degradation, showed high cellular infiltration, and produced the most cartilage-like tissue while having stiffer, less permeable material properties. Our results suggest that the in vivo biomaterial degradation rate is a dynamic property that can be optimized to influence cell fate and tissue repair in physeal injuries.